Hg2+ detection using a phosphorothioate RNA probe adsorbed on graphene oxide and a comparison with thymine-rich DNA
dc.contributor.author | Huang, Po-Jung Jimmy | |
dc.contributor.author | van Ballegooie, Courtney | |
dc.contributor.author | Liu, Juewen | |
dc.date.accessioned | 2017-04-28T16:11:58Z | |
dc.date.available | 2017-04-28T16:11:58Z | |
dc.date.issued | 2016-06-21 | |
dc.description.abstract | Mercury is a highly toxic heavy metal and many DNA-based biosensors have been recently developed for Hg2+ detection in water. Among them, thymine-rich DNA is the most commonly used for designing Hg2+ sensors. However, the thymine-Hg2+ interaction is strongly affected by the buffer conditions. We recently reported a molecular beacon containing phosphorothioate (PS)-modified RNA linkages that can be cleaved by Hg2+. In this work, the fluorescence quenching and DNA adsorption properties of nano-sized graphene oxide (NGO) were used to develop a new sensor using the PS-RNA chemistry. Three DNA probes, containing one, three and five PS-RNA linkages, respectively, were tested. Finally, a fluorophore-labeled poly-A DNA with five PS-RNA linkages was selected and adsorbed by NGO. In the presence of Hg2+, the fluorophore was released from NGO due to the cleavage reaction, resulting in a fluorescence enhancement. This sensor is highly selective for Hg2+ with a detection limit of 8.5 nM Hg2+. For comparison, a fluorophore-labeled poly-T DNA was also tested, which responded to Hg2+ more slowly and was inhibited by high NaCl concentrations, while the PS-RNA probe was more tolerant to different buffer conditions. This work indicates a new method for interfacing DNA with NGO for Hg2+ detection. | en |
dc.description.sponsorship | Natural Sciences and Engineering Research Council of Canada (NSERC) [386326, STPGP 447472-13] | en |
dc.identifier.uri | http://dx.doi.org/10.1039/c5an02031j | |
dc.identifier.uri | http://hdl.handle.net/10012/11795 | |
dc.language.iso | en | en |
dc.publisher | Royal Society of Chemistry | en |
dc.subject | Colorimetric Detection | en |
dc.subject | Fluorescent Sensors | en |
dc.subject | Aqueous-Solutions | en |
dc.subject | Optical-Detection | en |
dc.subject | Mercuric Ion | en |
dc.subject | DNAzyme | en |
dc.subject | Desulfurization | en |
dc.subject | Sensitivity | en |
dc.subject | Selectivity | en |
dc.subject | Mercury(II) | en |
dc.title | Hg2+ detection using a phosphorothioate RNA probe adsorbed on graphene oxide and a comparison with thymine-rich DNA | en |
dc.type | Article | en |
dcterms.bibliographicCitation | Huang, P.-J. J., van Ballegooie, C., & Liu, J. (2016). Hg2+ detection using a phosphorothioate RNA probe adsorbed on graphene oxide and a comparison with thymine-rich DNA. Analyst, 141(12), 3788–3793. https://doi.org/10.1039/c5an02031j | en |
uws.contributor.affiliation1 | Faculty of Science | en |
uws.contributor.affiliation2 | Chemistry | en |
uws.contributor.affiliation3 | Waterloo Institute for Nanotechnology (WIN) | en |
uws.peerReviewStatus | Reviewed | en |
uws.scholarLevel | Faculty | en |
uws.typeOfResource | Text | en |
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