A ring-polymer model shows how macromolecular crowding controls chromosome-arm organization in Escherichia coli

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dc.contributor.authorJeon, Chanil
dc.contributor.authorJung, Youngkyun
dc.contributor.authorHa, Bae-Yeun
dc.date.accessioned2017-11-07T16:22:18Z
dc.date.available2017-11-07T16:22:18Z
dc.date.issued2017-09-19
dc.description.abstractMacromolecular crowding influences various cellular processes such as macromolecular association and transcription, and is a key determinant of chromosome organization in bacteria. The entropy of crowders favors compaction of long chain molecules such as chromosomes. To what extent is the circular bacterial chromosome, often viewed as consisting of "two arms", organized entropically by crowding? Using computer simulations, we examine how a ring polymer is organized in a crowded and cylindrically-confined space, as a coarse-grained bacterial chromosome. Our results suggest that in a wide parameter range of biological relevance crowding is essential for separating the two arms in the way observed with Escherichia coli chromosomes at fast-growth rates, in addition to maintaining the chromosome in an organized collapsed state. Under different conditions, however, the ring polymer is centrally condensed or adsorbed onto the cylindrical wall with the two arms laterally collapsed onto each other. We discuss the relevance of our results to chromosome-membrane interactions.en
dc.description.sponsorshipKorea Institute of Science and Technology Information (KISTI)en
dc.description.sponsorshipNSERC (Canada)en
dc.description.sponsorshipBasic Science Research Program [2015R1D1A1A09057469]en
dc.identifier.urihttp://dx.doi.org/10.1038/s41598-017-10421-y
dc.identifier.urihttp://hdl.handle.net/10012/12614
dc.language.isoenen
dc.publisherNature Publishing Groupen
dc.rightsAttribution 4.0 International*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectSpatial-Organizationen
dc.subjectRna-Polymeraseen
dc.subjectBacterial Chromosomesen
dc.subjectMolecular-Dynamicsen
dc.subjectDnaen
dc.subjectConfinementen
dc.subjectConsequencesen
dc.subjectTranslationen
dc.subjectSegregationen
dc.subjectBiopolymeren
dc.titleA ring-polymer model shows how macromolecular crowding controls chromosome-arm organization in Escherichia colien
dc.typeArticleen
dcterms.bibliographicCitationJeon, C., Jung, Y., & Ha, B.-Y. (2017). A ring-polymer model shows how macromolecular crowding controls chromosome-arm organization in Escherichia coli. Scientific Reports, 7(1). https://doi.org/10.1038/s41598-017-10421-yen
uws.contributor.affiliation1Faculty of Scienceen
uws.contributor.affiliation2Physics and Astronomyen
uws.peerReviewStatusRevieweden
uws.scholarLevelFacultyen
uws.typeOfResourceTexten

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