Label-Free Ag+ Detection by Enhancing DNA Sensitized Tb3+ Luminescence
dc.contributor.author | Kleinke, Kimberly | |
dc.contributor.author | Saran, Runjhun | |
dc.contributor.author | Liu, Juewen | |
dc.date.accessioned | 2017-04-28T16:12:01Z | |
dc.date.available | 2017-04-28T16:12:01Z | |
dc.date.issued | 2016-08-26 | |
dc.description.abstract | In this work, the effect of Ag+ on DNA sensitized Tb3+ luminescence was studied initially using the Ag+-specific RNA-cleaving DNAzyme, Ag10c. While we expected to observe luminescence quenching by Ag+, a significant enhancement was produced. Based on this observation, simple DNA oligonucleotide homopolymers were used with systematically varied sequence and length. We discovered that both poly-G and poly-T DNA have a significant emission enhancement by Ag+, while the absolute intensity is stronger with the poly-G DNA, indicating that a G-quadruplex DNA is not required for this enhancement. Using the optimized length of the G7 DNA (an oligo constituted with seven guanines), Ag+ was measured with a detection limit of 57.6 nM. The signaling kinetics, G7 DNA conformation, and the binding affinity of Tb3+ to the DNA in the presence or absence of Ag+ are also studied to reveal the mechanism of emission enhancement. This observation is useful not only for label-free detection of Ag+, but also interesting for the rational design of new biosensors using Tb3+ luminescence. | en |
dc.description.sponsorship | Funding for this work is from the Natural Sciences and Engineering Research Council of Canada (NSERC). | en |
dc.identifier.uri | http://dx.doi.org/10.3390/s16091370 | |
dc.identifier.uri | http://hdl.handle.net/10012/11799 | |
dc.language.iso | en | en |
dc.publisher | Multidisciplinary Digital Publishing Institute | en |
dc.rights | Attribution 4.0 International | * |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Coordination Polymers | en |
dc.subject | Silver Nanoparticles | en |
dc.subject | Metal-Ions | en |
dc.subject | Binding | en |
dc.subject | Fluorescence | en |
dc.subject | Sequence | en |
dc.subject | Dnazyme | en |
dc.subject | Nanoclusters | en |
dc.subject | Molecules | en |
dc.subject | Duplexes | en |
dc.title | Label-Free Ag+ Detection by Enhancing DNA Sensitized Tb3+ Luminescence | en |
dc.type | Article | en |
dcterms.bibliographicCitation | Kleinke, K., Saran, R., & Liu, J. (2016). Label-Free Ag+ Detection by Enhancing DNA Sensitized Tb3+ Luminescence. Sensors, 16(9), 1370. https://doi.org/10.3390/s16091370 | en |
uws.contributor.affiliation1 | Faculty of Science | en |
uws.contributor.affiliation2 | Chemistry | en |
uws.contributor.affiliation3 | Waterloo Institute for Nanotechnology (WIN) | en |
uws.peerReviewStatus | Reviewed | en |
uws.scholarLevel | Faculty | en |
uws.typeOfResource | Text | en |