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Porous chitosan microspheres as microcarriers for 3D cell culture

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dc.contributor.authorHuang, Lixia
dc.contributor.authorXiao, Lin
dc.contributor.authorJung Poudel, Abishek
dc.contributor.authorLi, Jixiang
dc.contributor.authorZhou, Ping
dc.contributor.authorGauthier, Mario
dc.contributor.authorLiu, Haiqing
dc.contributor.authorWu, Zhihong
dc.contributor.authorYang, Guang
dc.date.accessioned2018-09-21T14:50:23Z
dc.date.available2018-09-21T14:50:23Z
dc.date.issued2018-12-15
dc.descriptionThe final publication is available at Elsevier via https://doi.org/10.1016/j.carbpol.2018.09.021 © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.description.abstractHighly porous chitosan microspheres (CSM) were prepared through emulsion-based thermally induced phase separation (TIPS) without using toxic crosslinkers and chemical porogenic agents other than ice. The CSM had an average diameter of ∼150 μm with interconnected pores varying from 20∼50 μm in size. Due to their excellent biocompatibility and unique porous structure, high-performance hepatocyte culture in three-dimensional (3D) space was achieved using the CSM as microcarriers, as cell growth also took place within the internal pores of the CSM, besides their external surface, and multidirectional cell–cell interactions were observed. Enhanced cellular activity and functions were obtained with the CSM microcarriers as compared with 2D cell culture. It is believed that these CSM microcarriers provide a promising platform for 3D cell culture in vitro.en
dc.description.sponsorshipNational Natural Science Foundation of China General Program (Nos. 51803067, 21574050 and 21774039) China Postdoctoral Science Foundation (General Program, No. 2015M580640) Science and Technology Support Plan in Jiangsu Province, China (BE2014684) Independent innovation research funding of Huazhong University of Science and Technology (2014XJGH009)en
dc.identifier.urihttps://doi.org/10.1016/j.carbpol.2018.09.021
dc.identifier.urihttp://hdl.handle.net/10012/13887
dc.language.isoenen
dc.publisherElsevieren
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subject3D cell cultureen
dc.subjectChitosanen
dc.subjectHepatocytesen
dc.subjectMicrocarriersen
dc.subjectPorous microspheresen
dc.titlePorous chitosan microspheres as microcarriers for 3D cell cultureen
dc.typeArticleen
dcterms.bibliographicCitationHuang, L., Xiao, L., Jung Poudel, A., Li, J., Zhou, P., Gauthier, M., … Yang, G. (2018). Porous chitosan microspheres as microcarriers for 3D cell culture. Carbohydrate Polymers, 202, 611–620. https://doi.org/10.1016/j.carbpol.2018.09.021en
uws.contributor.affiliation1Faculty of Scienceen
uws.contributor.affiliation2Chemistryen
uws.peerReviewStatusRevieweden
uws.scholarLevelFacultyen
uws.typeOfResourceTexten

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