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Homogeneous assays for aptamer-based ethanolamine sensing: no indication of target binding

dc.contributor.authorDing, Yuzhe
dc.contributor.authorLiu, Xun
dc.contributor.authorHuang, Po-Jung Jimmy
dc.contributor.authorLiu, Juewen
dc.date.accessioned2025-09-17T13:36:14Z
dc.date.available2025-09-17T13:36:14Z
dc.date.issued2022-02-11
dc.description.abstractEthanolamine is an important analyte for environmental chemistry and biological sciences. A few DNA aptamers were previously reported for binding ethanolamine with a dissociation constant (Kd) as low as 9.6 nM. However, most of the previous binding assays and sensing work used either immobilized ethanolamine or immobilized aptamers. In this work, we studied three previously reported DNA sequences, two of which were supposed to bind ethanolamine while the other could not bind. Isothermal titration calorimetry revealed no binding for any of these sequences. In addition, due to their guanine-rich sequences, thioflavin T was used as a probe. Little fluorescence change was observed with up to 1 μM ethanolamine. Responses within the millimolar range of ethanolamine were attributed to the general fluorescence quenching effect of ethanolamine instead of aptamer binding. Finally, after studying the adsorption of ethanolamine to gold nanoparticles (AuNPs), we confirmed the feasibility of using AuNPs as a probe when the concentration of ethanolamine was below 0.1 mM. However, no indication of specific aptamer binding was observed by comparing the three DNA sequences for their color changing trends. This work articulates the importance of careful homogeneous binding assays using free target molecules.
dc.identifier.urihttps://doi.org/10.1039/d2an00145d
dc.identifier.uri10.1039/D2AN00145D
dc.identifier.urihttps://hdl.handle.net/10012/22451
dc.language.isoen
dc.publisherRoyal Society of Chemistry
dc.relation.ispartofseriesAnalyst; 147(7)
dc.rightsAttribution-NonCommercial-ShareAlike 2.5 Canadaen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/2.5/ca/
dc.titleHomogeneous assays for aptamer-based ethanolamine sensing: no indication of target binding
dc.typeArticle
dcterms.bibliographicCitationDing, Y., Liu, X., Huang, P.-J. J., & Liu, J. (2022). Homogeneous assays for aptamer-based ethanolamine sensing: No indication of target binding. The Analyst, 147(7), 1348–1356. https://doi.org/10.1039/d2an00145d
uws.contributor.affiliation1Faculty of Science
uws.contributor.affiliation2Chemistry
uws.peerReviewStatusReviewed
uws.scholarLevelFaculty
uws.typeOfResourceTexten

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