CK-2 of rainbow trout (Oncorhynchus mykiss) has two differentially regulated alleles that encode a functional chemokine
dc.contributor.author | Semple, Shawna L. | |
dc.contributor.author | Eshaque, Shathi | |
dc.contributor.author | Fujiki, Kazuhiro | |
dc.contributor.author | Tang, Cynthia | |
dc.contributor.author | Mitchell, Leslie | |
dc.contributor.author | Bols, Niels C. | |
dc.contributor.author | Dixon, Brian | |
dc.date.accessioned | 2018-07-27T15:14:03Z | |
dc.date.available | 2018-07-27T15:14:03Z | |
dc.date.issued | 2018-04 | |
dc.description | The final publication is available at Elsevier via https://doi.org/10.1016/j.vetimm.2018.02.003. © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | en |
dc.description.abstract | Rainbow trout chemokine 2 (CK-2) is currently the only known CC chemokine to have a mucin stalk. Further analysis of the mucin stalk region revealed a second, related CC chemokine sequence, denoted here as CK-2.1. This second sequence was determined to be an allele of CK-2 following genomic PCR analysis on several outbred individuals. Furthermore, in both in vivo and in vitro trials, CK-2 and CK-2.1 were both present, but appeared to have differential tissue expression in both control and PHA stimulated samples. Upon the development of a polyclonal antibody to rCK-2, CK-2 was only observed in the brain, liver and head kidney of PHA stimulated rainbow trout tissues. In comparison, when using the rainbow trout monocyte/macrophage-like cell line, RTS-11, CK-2 protein was observed in both control and PHA stimulated conditions. When studying the function of CK-2, a chemotaxis assay revealed that both peripheral blood leukocytes and RTS-11 cells migrated towards rCK-2 significantly at all concentrations studied when compared to truncated β2m. Interestingly, this migration was lowest at both the highest concentration and the lowest concentrations of CK-2. Thus, teleostean chemokine receptors may become desensitized when overstimulated as has been observed in mammalian models. The observed chemotactic function was indeed due to rCK-2 as cell migration was inhibited through pre-treatment of both the cells and the polyclonal antibody with rCK-2. As has been observed thus far with all other chemokines, CK-2 does appear to function through binding to a G-coupled protein receptor as chemotaxis could be inhibited through pre-treatment with pertussis toxin. Overall, the results of this study indicate that CK-2 is a functional chemokine that is encoded by two differentially expressed alleles in rainbow trout, CK-2 and CK-2.1. | en |
dc.description.sponsorship | Natural Sciences and Engineering Research Council || 217529 | en |
dc.identifier.issn | 0165-2427 | |
dc.identifier.uri | https://dx.doi.org/10.1016/j.vetimm.2018.02.003 | |
dc.identifier.uri | http://hdl.handle.net/10012/13495 | |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | phytohemagglutinin (PHA) | en |
dc.subject | peripheral blood leukocytes | en |
dc.subject | rainbow trout | en |
dc.subject | chemotaxis | en |
dc.subject | CC chemokine | en |
dc.title | CK-2 of rainbow trout (Oncorhynchus mykiss) has two differentially regulated alleles that encode a functional chemokine | en |
dc.type | Article | en |
dcterms.bibliographicCitation | Semple, S. L., Eshaque, S., Fujiki, K., Tang, C., Mitchell, L., Bols, N. C., & Dixon, B. (2018). CK-2 of rainbow trout (Oncorhynchus mykiss) has two differentially regulated alleles that encode a functional chemokine. Veterinary Immunology and Immunopathology, 198, 26–36. https://doi.org/10.1016/j.vetimm.2018.02.003 | en |
uws.contributor.affiliation1 | Faculty of Science | en |
uws.contributor.affiliation2 | Biology | en |
uws.peerReviewStatus | Reviewed | en |
uws.scholarLevel | Faculty | en |
uws.scholarLevel | Graduate | en |
uws.typeOfResource | Text | en |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- 1-s2.0-S0165242717305494-main.pdf
- Size:
- 1005.24 KB
- Format:
- Adobe Portable Document Format
- Description:
- Post-print
License bundle
1 - 1 of 1
No Thumbnail Available
- Name:
- license.txt
- Size:
- 6.08 KB
- Format:
- Item-specific license agreed upon to submission
- Description: