The Libraries will be performing routine maintenance on UWSpace on October 13th, 2025, from 8 - 9 am ET. UWSpace will be unavailable during this time. Service should resume by 9 am ET.
 

Signaling Kinetics of DNA and Aptamer Biosensors Revealing Graphene Oxide Surface Heterogeneity

dc.contributor.authorHuang, Po-Jung Jimmy
dc.contributor.authorLiu, Juewen
dc.date.accessioned2025-09-22T15:00:39Z
dc.date.available2025-09-22T15:00:39Z
dc.date.issued2021-12-16
dc.descriptionThis is a post-peer-review, pre-copyedit version of an article published in Journal of Analysis and Testing. The final authenticated version is available online at: https://doi.org/10.1007/s41664-021-00201-z
dc.description.abstractAdsorption of fluorescently labeled DNA and aptamer probes to graphene oxide (GO) has been one of the most popular methods for developing biosensors. In the presence of target analytes, the quenched fluorescence would recover. In this work, we followed the kinetics of the reactions and found that the fluorescence would eventually drop after an initial increase, and this was attributed to the re-adsorption of the desorbed probe DNA molecules. Both a DNA probe and an aptamer for adenosine were used. This re-adsorption was attributed to the surface heterogeneity of GO, and the DNA probes desorbed from relatively weaker binding sites were re-adsorbed on the stronger binding sites. This re-adsorption can be avoided by extensive washing the samples, and also by blocking the GO surface or by heating. This fundamental understanding is important for achieving a stable signal of such biosensors.
dc.identifier.urihttps://doi.org/10.1007/s41664-021-00201-z
dc.identifier.uri10.1007/s41664-021-00201-z
dc.identifier.urihttps://hdl.handle.net/10012/22504
dc.language.isoen
dc.publisherSpringer Nature
dc.relation.ispartofseriesJournal of Analytics and Testing; 6(1)
dc.rightsAttribution-NonCommercial-ShareAlike 2.5 Canadaen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/2.5/ca/
dc.titleSignaling Kinetics of DNA and Aptamer Biosensors Revealing Graphene Oxide Surface Heterogeneity
dc.typeArticle
dcterms.bibliographicCitationHuang, P.-J. J., & Liu, J. (2021). Signaling kinetics of DNA and aptamer biosensors revealing graphene oxide surface heterogeneity. Journal of Analysis and Testing, 6(1), 20–27. https://doi.org/10.1007/s41664-021-00201-z
uws.contributor.affiliation1Faculty of Science
uws.contributor.affiliation2Chemistry
uws.peerReviewStatusReviewed
uws.scholarLevelFaculty
uws.typeOfResourceTexten

Files

Original bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
intriguing aptamer kinetics-5 for JOAT-revised2.pdf
Size:
1.49 MB
Format:
Adobe Portable Document Format

License bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
4.47 KB
Format:
Item-specific license agreed upon to submission
Description: