Browsing Science (Faculty of) by Supervisor "Dieckmann, Thorsten"
Now showing items 1-6 of 6
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Biochemical Characterization and Optimization of RNA Aptamers Bound to Hoechst Dye Derivatives
(University of Waterloo, 2023-08-09)Ribonucleic acid (RNA) aptamers are single-stranded nucleic acids that are typically selected through a process known as Systematic Evolution of Ligands by EXponential Enrichment (SELEX). These oligonucleotides can bind ... -
Biochemical Characterization of Fluorophore-Binding Nucleic Acids
(University of Waterloo, 2022-09-27)Aptamers, single stranded RNA or DNA that bind to target ligands with high affinity and specificity, have a wide range of applications, from therapeutics to biosensing, which makes biochemical characterization of the utmost ... -
Determining the Structures and Properties of Biologically-relevant Ions
(University of Waterloo, 2018-08-09)Gas phase studies of biologically relevant ions are increasing in popularity due to the possibility of high throughput analysis requiring minimum sample concentrations. This thesis explores the potential of differential ... -
Investigation of the Presence of a G-quadruplex In the Hoechst Dye Binding DNA Aptamer
(University of Waterloo, 2022-04-22)Aptamers are nucleic acid sequences that have been selected to bind to a ligand with high affinity and selectivity. Aptamers have great potential for use in various biotechnological and medical applications but understanding ... -
NMR Studies of Protein and Peptide Structure and Dynamics
(University of Waterloo, 2016-04-18)Calmodulin (CaM) is a small, acidic cytosolic calcium binding protein that responds to increases in intracellular Ca2+ concentrations. It is proposed to be involved in binding to and regulating over 300 functionally and ... -
Selection and characterization of an aptamer to a thiazole orange derivative for use in a fluorescence-based biosensor
(University of Waterloo, 2023-08-18)Aptamers are short single-stranded DNA or RNA sequences that have been selected in vitro to have a high binding affinity to a specific target molecule. One class of aptamers can bind to small non-fluorescent molecules to ...