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dc.contributor.authorCourtney, Matthew
dc.contributor.authorChen, Xiaoming
dc.contributor.authorChan, Sarah
dc.contributor.authorMohamed, Tarek
dc.contributor.authorRao, Praveen P.N.
dc.contributor.authorRen, Carolyn L. 15:42:38 (GMT) 15:42:38 (GMT)
dc.descriptionThis document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, copyright © American Chemical Society after peer review and technical editing by publisher. To access the final edited and published work see
dc.description.abstract96-Well plate has been the traditional method used for screening drug compounds libraries for potential bioactivity. Although this method has been proven successful in testing doseÐresponse analysis, the microliter consumption of expensive reagents and hours of reaction and analysis time call for innovative methods for improvements. This work demonstrates a droplet microfluidic platform that has the potential to significantly reduce the reagent consumption and shorten the reaction and analysis time by utilizing nanoliter-sized droplets as a replacement of wells. This platform is evaluated by applying it to screen drug compounds that inhibit the tau-peptide aggregation, a phenomena related to AlzheimerÕs disease. In this platform, sample reagents are first dispersed into nanolitre-sized droplets by an immiscible carrier oil and then these droplets are trapped on-demand in the downstream of the microfluidic device. The relative decrease in fluorescence through drug inhibition is characterized using an inverted epifluorescence microscope. Finally, the trapped droplets are released on-demand after each test by manipulating the applied pressures to the channel network which allows continuous processing. The testing results agree well with that obtained from 96-well plates with much lower sample consumption (_200 times lower than 96-well plate) and reduced reaction time due to increased surface volume ratio (2.5 min vs 2 h).en
dc.description.sponsorshipNatural Science and Engineering Research Council of Canadaen
dc.description.sponsorshipCanada Research Chair programen
dc.description.sponsorshipCanada Foundation for Innovationen
dc.description.sponsorshipUniversity of Waterlooen
dc.description.sponsorshipOntario Mental Health Foundationen
dc.description.sponsorshipNSERC-Discovery grant RGPIN 03830-2014en
dc.description.sponsorshipMinistry of Research and Innovation, Government of Ontarioen
dc.publisherAmerican Chemical Society (ACS)en
dc.titleDroplet Microfluidic System with On-Demand Trapping and Releasing of Droplet for Drug Screening Applicationsen
dcterms.bibliographicCitationCourtney, M., Chen, X., Chan, S., Mohamed, T., Rao, P. P. N., & Ren, C. L. (2017). Droplet Microfluidic System with On-Demand Trapping and Releasing of Droplet for Drug Screening Applications. Analytical Chemistry, 89(1), 910Ð915.
uws.contributor.affiliation1Faculty of Engineeringen
uws.contributor.affiliation1Faculty of Scienceen
uws.contributor.affiliation2Mechanical and Mechatronics Engineeringen
uws.contributor.affiliation2School of Pharmacyen
uws.contributor.affiliation2Nanotechnology Engineeringen

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