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dc.contributor.authorSemple, Shawna L.
dc.contributor.authorVo, Nguyen T. K.
dc.contributor.authorPoynter, Sarah J.
dc.contributor.authorLi, M.
dc.contributor.authorHeath, Daniel D.
dc.contributor.authorDeWitte-Orr, Stephanie J.
dc.contributor.authorDixon, Brian 16:43:24 (GMT) 16:43:24 (GMT)
dc.descriptionThe final publication is available at Elsevier via © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
dc.description.abstractDespite increased global interest in Chinook salmon aquaculture, little is known of their viral immune defenses. This study describes the establishment and characterization of a continuous cell line derived from Chinook salmon spleen, CHSS, and its use in innate immune studies. Optimal growth was seen at 14–18 °C when grown in Leibovitz's L-15 media with 20% fetal bovine serum. DNA analyses confirmed that CHSS was Chinook salmon and genetically different from the only other available Chinook salmon cell line, CHSE-214. Unlike CHSE-214, CHSS could bind extracellular dsRNA, resulting in the rapid and robust expression of antiviral genes. Receptor/ligand blocking assays confirmed that class A scavenger receptors (SR-A) facilitated dsRNA binding and subsequent gene expression. Although both cell lines expressed three SR-A genes: SCARA3, SCARA4, and SCARA5, only CHSS appeared to have functional cell-surface SR-As for dsRNA. Collectively, CHSS is an excellent cell model to study dsRNA-mediated innate immunity in Chinook salmon.en
dc.description.sponsorshipNatural Sciences and Engineering Research Council of Canadaen
dc.description.sponsorshipCanada Research Councilen
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.subjectCell lineen
dc.subjectChinook salmonen
dc.subjectInnate immunityen
dc.subjectInterferon-stimulated genesen
dc.subjectpolyinosinic:polycytidylic aciden
dc.titleExtracellular dsRNA induces a type I interferon response mediated via class A scavenger receptors in a novel Chinook salmon derived spleen cell lineen
dcterms.bibliographicCitationSemple, S. L., Vo, N. T. K., Poynter, S. J., Li, M., Heath, D. D., DeWitte-Orr, S. J., & Dixon, B. (2018). Extracellular dsRNA induces a type I interferon response mediated via class A scavenger receptors in a novel Chinook salmon derived spleen cell line. Developmental & Comparative Immunology, 89, 93–101. doi:10.1016/j.dci.2018.08.010en
uws.contributor.affiliation1Faculty of Scienceen

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