Consequences of cytochrome c oxidase assembly defects for the yeast stationary phase
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Date
2018-06-01
Authors
Dubinski, Alicia F.
Camasta, Raffaele
Soule, Tyler G. B.
Reed, Bruce H.
Glerum, D. Moira
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
The assembly of cytochrome c oxidase (COX) is essential for a functional mitochondrial respiratory chain, although the consequences of a loss of assembled COX at yeast stationary phase, an excellent model for terminally differentiated cells in humans, remain largely unexamined. In this study, we show that a wild-type respiratory competent yeast strain at stationary phase is characterized by a decreased oxidative capacity, as seen by a reduction in the amount of assembled COX and by a decrease in protein levels of several COX assembly factors. In contrast, loss of assembled COX results in the decreased abundance of many mitochondrial proteins at stationary phase, which is likely due to decreased membrane potential and changes in mitophagy. In addition to an altered mitochondrial proteome, COX assembly mutants display unexpected changes in markers of cellular oxidative stress at stationary phase. Our results suggest that mitochondria may not be a major source of reactive oxygen species at stationary phase in cells lacking an intact respiratory chain.
Description
The final publication is available at Elsevier via http://dx.doi.org/10.1016/j.bbabio.2018.03.011 © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
Keywords
Cytochrome oxidase (complex IV) assembly, Mitochondria, Oxidative stress, Stationary phase, Yeast