dc.contributor.author | Ma, Tianchi | |
dc.contributor.author | Northrup, Victoria | |
dc.contributor.author | Fung, Andrew O. | |
dc.contributor.author | Glerum, D. Moira | |
dc.contributor.author | Backhouse, Christopher J. | |
dc.date.accessioned | 2018-04-20 16:15:04 (GMT) | |
dc.date.available | 2018-04-20 16:15:04 (GMT) | |
dc.date.issued | 2012-10-24 | |
dc.identifier.uri | http://dx.doi.org/10.1117/12.2001470 | |
dc.identifier.uri | http://hdl.handle.net/10012/13144 | |
dc.description | Tianchi Ma, Victoria Northrup, Andrew O. Fung, D. Moira Glerum, Christopher J. Backhouse, "Polymeric rapid prototyping for inexpensive and portable medical diagnostics", Proc. SPIE 8412, Photonics North 2012, 84120B (24 October 2012); doi: 10.1117/12.2001470. Copyright 2012, Society of Photo Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. https://doi.org/10.1117/12.2001470 | en |
dc.description.abstract | The advent of inexpensive CO2 laser systems has led to a wide range of demonstrations of microfabricated lab on chip systems built of acrylic. However, there has been little application of these systems to building microfluidics for DNA analysis. In this work we explore the use of CO2 laser systems for building microfluidics for DNA analysis and relate the artifacts of the fabrication technology to the performance of the system. We show that surface roughness that leads to significant constrictions in the separation channel provides an upper limit of the size of DNA that can be analysed. Below that upper limit, the resolution of the chip is strongly affected by the degree to which the separation channel is exposed to redeposited by-products of the ablation process. We show that by controlling these effects we are reliably able to discern two types of PCR product as a test representative of a real application. By being able to do this is in microfluidic devices the size of a postage stamp we have shown that we can now use CO2 laser systems for the development of extremely inexpensive diagnostic systems using a rapid prototyping approach. | en |
dc.description.sponsorship | Natural Sciences and Engineering Research Council of Canada | en |
dc.description.sponsorship | Teledyne-DALSA | en |
dc.language.iso | en | en |
dc.publisher | Society of Photo-optical Instrumentation Engineers | en |
dc.subject | Electrophoresis | en |
dc.subject | Acrylic | en |
dc.subject | PMMA | en |
dc.subject | DNA | en |
dc.subject | separation | en |
dc.title | Polymeric rapid prototyping for inexpensive and portable medical diagnostics | en |
dc.type | Conference Paper | en |
dcterms.bibliographicCitation | Tianchi Ma, Victoria Northrup, Andrew O. Fung, D. Moira Glerum, Christopher J. Backhouse, "Polymeric rapid prototyping for inexpensive and portable medical diagnostics", Proc. SPIE 8412, Photonics North 2012, 84120B (24 October 2012); doi: 10.1117/12.2001470; https://doi.org/10.1117/12.2001470 | en |
uws.contributor.affiliation1 | Faculty of Science | en |
uws.contributor.affiliation2 | Biology | en |
uws.typeOfResource | Text | en |
uws.typeOfResource | Text | en |
uws.peerReviewStatus | Reviewed | en |
uws.scholarLevel | Faculty | en |