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dc.contributor.authorMardian, Emily B.
dc.contributor.authorBradley, Ryan M.
dc.contributor.authorHenao, Juan J. Aristizabal
dc.contributor.authorMarvyn, Phillip M.
dc.contributor.authorMoes, Katherine A.
dc.contributor.authorBombardier, Eric
dc.contributor.authorTupling, A. Russell
dc.contributor.authorStark, Ken
dc.contributor.authorDuncan, Robin E.
dc.date.accessioned2017-11-20 18:53:23 (GMT)
dc.date.available2017-11-20 18:53:23 (GMT)
dc.date.issued2017-10-01
dc.identifier.urihttp:/dx.doi.org/10.1194/jlr.M079152
dc.identifier.urihttp://hdl.handle.net/10012/12637
dc.descriptionThis research was originally published in Journal of Lipid Research. Mardian, E. B., Bradley, R. M., Aristizabal Henao, J. J., Marvyn, P. M., Moes, K. A., Bombardier, E., … Duncan, R. E.. Agpat4/Lpaatδ deficiency highlights the molecular heterogeneity of epididymal and perirenal white adipose depots. Journal of Lipid Research. 2017; 58(10), 2037–2050 © the American Society for Biochemistry and Molecular Biology.en
dc.description.abstractAcylglycerophosphate acyltransferase 4 (AGPAT4)/lysophosphatidic acid acyltransferase delta catalyzes the formation of phosphatidic acid (PA), a precursor of triacylglycerol (TAG). We investigated the effect of Agpat4 gene ablation on white adipose tissue (WAT) after finding consistent expression across depots. Epididymal WAT mass was 40% larger in male Agpat4(-/-) mice than wild-type littermates, but unchanged in perirenal, retroperitoneal, and inguinal WAT and subscapular brown adipose tissue. Metabolic changes were identified in epididymal WAT that were not evident in perirenal WAT, which was analyzed for comparison. The total epididymal TAG content doubled, increasing adipocyte cell size without changing markers of differentiation. Enzymes involved in de novo lipogenesis and complex lipid synthesis downstream of phosphatidic acid production were also unchanged. However, total epididymal TAG hydrolase activity was reduced, and there were significant decreases in total ATGL and reduced phosphorylation of hormone-sensitive lipase at the S563 and S660 PKA-activation sites. Analysis of Agpats 1, 2, 3, and 5, as well as Gpats 1, 2, 3, and 4, demonstrated compensatory upregulation in perirenal WAT that did not occur in epididymal WAT. Our findings therefore indicate depot-specific differences in the redundancy of Agpat4 and highlight the molecular and metabolic heterogeneity of individual visceral depots.en
dc.description.sponsorshipCanada Foundation for Innovation [30259]en
dc.description.sponsorshipNatural Sciences and Engineering Research Council (NSERC) of Canada [418213-2012]en
dc.description.sponsorshipCanada Research Chair in Nutritional Lipidomicsen
dc.description.sponsorshipNSERC Canada Graduate Scholarship-Master's (CGS-M)en
dc.description.sponsorshipNSERC Postgraduate Scholarships-Doctoral (PGS-D)en
dc.language.isoenen
dc.publisherAmerican Society for Biochemistry and Molecular Biologyen
dc.subjectAcylglycerophosphate Acyltransferase 4en
dc.subjectAdipose Tissueen
dc.subjectLipid Biochemistryen
dc.subjectLipolysis And Fatty Acid Metabolismen
dc.subjectPhospholipids/Phosphatidic Aciden
dc.subjectTriglyceridesen
dc.subjectLipogenesisen
dc.subjectLysophosphatidic Acid Acyltransferaseen
dc.titleAgpat4/Lpaat delta deficiency highlights the molecular heterogeneity of epididymal and perirenal white adipose depotsen
dc.typeArticleen
dcterms.bibliographicCitationMardian, E. B., Bradley, R. M., Aristizabal Henao, J. J., Marvyn, P. M., Moes, K. A., Bombardier, E., … Duncan, R. E. (2017). Agpat4/Lpaatδ deficiency highlights the molecular heterogeneity of epididymal and perirenal white adipose depots. Journal of Lipid Research, 58(10), 2037–2050. https://doi.org/10.1194/jlr.M079152en
uws.contributor.affiliation1Faculty of Applied Health Sciencesen
uws.contributor.affiliation2Kinesiologyen
uws.typeOfResourceTexten
uws.peerReviewStatusRevieweden
uws.scholarLevelFacultyen


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