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A new and efficient Solid Phase Microextraction approach for analysis of high fat content food samples using a matrix-compatible coating

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Date

2017-05-15

Authors

De Grazia, Selenia
Gionfriddo, Emanuela
Pawliszyn, Janusz

Journal Title

Journal ISSN

Volume Title

Publisher

Elsevier

Abstract

The current work presents the optimization of a protocol enabling direct extraction of avocado samples by a new Solid Phase Microextraction matrix compatible coating. In order to further extend the coating life time, pre-desorption and post-desorption washing steps were optimized for solvent type, time, and degree of agitation employed. Using optimized conditions, lifetime profiles of the coating related to extraction of a group of analytes bearing different physical-chemical properties were obtained. Over 80 successive extractions were carried out to establish coating efficiency using PDMS/DVB 65 mu m commercial coating in comparison with the PDMS/DVB/ PDMS. The PDMS/DVB coating was more prone to irreversible matrix attachment on its surface, with consequent reduction of its extractive performance after 80 consecutive extractions. Conversely, the PDMS/DVB/PDMS coating showed enhanced inertness towards matrix fouling due to its outer smooth PDMS layer. This work represents the first step towards the development of robust SPME methods for quantification of contaminants in avocado as well as other fatty-based matrices, with minimal sample pre-treatment prior to extraction. In addition, an evaluation of matrix components attachment on the coating surface and related artifacts created by desorption of the coating at high temperatures in the GC-injector port, has been performed by GCxGC-ToF/MS.

Description

The final publication is available at Elsevier via http://dx.doi.org/10.1016/j.talanta.2017.01.064 © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/

Keywords

Matrix-compatible SPME coating, Direct immersion, Avocado, Extended lifetime, PDMS/DVB/PDMS

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Citation

URI

http://dx.doi.org/10.1016/j.talanta.2017.01.064
 http://hdl.handle.net/10012/12541

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Waterloo Research
Chemistry