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dc.contributor.authorYen, Sandi
dc.contributor.authorSokolenko, Stanislav
dc.contributor.authorManocha, Bhavik
dc.contributor.authorBlondeel, Eric J. M.
dc.contributor.authorAucoin, Marc
dc.contributor.authorPatras, Ankit
dc.contributor.authorDaynouri-Pancino, Farnaz
dc.contributor.authorSasges, Michael
dc.date.accessioned2017-06-05 14:28:42 (GMT)
dc.date.available2017-06-05 14:28:42 (GMT)
dc.date.issued2014-10-01
dc.identifier.urihttp://dx.doi.org/10.1002/btpr.1942
dc.identifier.urihttp://hdl.handle.net/10012/11984
dc.description.abstractSterility of cell culture media is an important concern in biotherapeutic processing. In large scale biotherapeutic production, a unit contamination of cell culture media can have costly effects. Ultraviolet (UV) irradiation is a sterilization method effective against bacteria and viruses while being non-thermal and non-adulterating in its mechanism of action. This makes UV irradiation attractive for use in sterilization of cell culture media. The objective of this study was to evaluate the effect of UV irradiation of cell culture media in terms of chemical composition and the ability to grow cell cultures in the treated media. The results showed that UV irradiation of commercial cell culture media at relevant disinfection doses impacted the chemical composition of the media with respect to several carboxylic acids, and to a minimal extent, amino acids. The cumulative effect of these changes, however, did not negatively influence the ability to culture Chinese Hamster Ovary cells, as evaluated by cell viability, growth rate, and protein titer measurements in simple batch growth compared with the same cells cultured in control media exposed to visible light. (C) 2014 American Institute of Chemical Engineersen
dc.description.sponsorshipNSERC ENGAGE; NSERC Strategic Network (MabNet); NSERC Canada Graduate Scholarshipen
dc.language.isoenen
dc.publisherWileyen
dc.rightsAttribution 3.0 Unported*
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/*
dc.subjectUltraviolet-Irradiationen
dc.subjectMinute Virusen
dc.subjectInactivationen
dc.subjectRadiationen
dc.subjectSterilizationen
dc.subjectBacteriaen
dc.subjectIndustryen
dc.titleTreating Cell Culture Media with UV Irradiation against Adventitious Agents: Minimal Impact on CHO Performanceen
dc.typeArticleen
dcterms.bibliographicCitationYen, S., Sokolenko, S., Manocha, B., Patras, A., Daynouri-Pancino, F., Blondeel, E. J. M., … Aucoin, M. G. (2014). Treating cell culture media with UV irradiation against adventitious agents: Minimal impact on CHO performance. Biotechnology Progress, 30(5), 1190–1195. https://doi.org/10.1002/btpr.1942en
uws.contributor.affiliation1Faculty of Engineeringen
uws.contributor.affiliation2Chemical Engineeringen
uws.typeOfResourceTexten
uws.typeOfResourceTexten
uws.peerReviewStatusRevieweden
uws.scholarLevelFacultyen


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Attribution 3.0 Unported
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