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dc.contributor.authorLu, Chang
dc.contributor.authorHuang, Po-Jung Jimmy
dc.contributor.authorLiu, Biwu
dc.contributor.authorYing, Yibin
dc.contributor.authorLiu, Juewen
dc.date.accessioned2017-04-28 16:12:06 (GMT)
dc.date.available2017-04-28 16:12:06 (GMT)
dc.date.issued2016-10-18
dc.identifier.urihttp://dx.doi.org/10.1021/acs.langmuir.6b03032
dc.identifier.urihttp://hdl.handle.net/10012/11809
dc.descriptionThis document is the Accepted Manuscript version of a Published Work that appeared in final form in Langmuir, © 2016 American Chemical Society after peer review and technical editing by publisher. To access the final edited and published work see Lu, C., Huang, P.-J. J., Liu, B., Ying, Y., & Liu, J. (2016). Comparison of Graphene Oxide and Reduced Graphene Oxide for DNA Adsorption and Sensing. Langmuir, 32(41), 10776–10783. https://doi.org/10.1021/acs.langmuir.6b03032en
dc.description.abstractFluorescently labeled DNA adsorbed on graphene oxide (GO) is a well-established sensing platform for detecting a diverse range of analytes. GO is a loosely defined material and its oxygen content may vary depending on the condition of preparation. Sometimes, a further reduction step is intentionally performed to decrease the oxygen content, and the resulting material is called reduced GO (rGO). In this study, DNA adsorption and desorption from GO and rGO is systematically compared. Under the same salt concentration, DNA adsorbs slightly faster with a 2.6-fold higher capacity on rGO. At the same time, DNA adsorbed on rGO is more resistant to desorption induced by temperature, pH, urea, and organic solvents. Various lengths and sequences of DNA probes have been tested. When its complementary DNA is added as a model target analyte, the rGO sample has a higher signal-to-background and signal-to-noise ratio, whereas the GO sample has a slightly higher absolute signal increase and faster signaling kinetics. DNAs adsorbed on GO or rGO are still susceptible to nonspecific, displacement by other DNA and proteins. Overall, although rGO adsorbs DNA more tightly, it allows efficient DNA sensing with an extremely low background fluorescence signal.en
dc.description.sponsorshipNatural Sciences and Engineering Research Council of Canada (NSERC); Ministry of Education of China [20120101130009]en
dc.language.isoenen
dc.publisherAmerican Chemical Societyen
dc.subjectSingle-Stranded-DNAen
dc.subjectPlatformen
dc.subjectFluorescenceen
dc.subjectSurfaceen
dc.subjectBiosensoren
dc.subjectAssayen
dc.subjectNanoparticlesen
dc.subjectSpectroscopyen
dc.subjectDesorptionen
dc.subjectGraphiteen
dc.titleComparison of Graphene Oxide and Reduced Graphene Oxide for DNA Adsorption and Sensingen
dc.typeArticleen
dcterms.bibliographicCitationLu, C., Huang, P.-J. J., Liu, B., Ying, Y., & Liu, J. (2016). Comparison of Graphene Oxide and Reduced Graphene Oxide for DNA Adsorption and Sensing. Langmuir, 32(41), 10776–10783. https://doi.org/10.1021/acs.langmuir.6b03032en
uws.contributor.affiliation1Faculty of Scienceen
uws.contributor.affiliation2Chemistryen
uws.contributor.affiliation3Waterloo Institute for Nanotechnology (WIN)en
uws.typeOfResourceTexten
uws.peerReviewStatusRevieweden
uws.scholarLevelFacultyen


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