A New Na+-Dependent RNA-Cleaving DNAzyme with over 1000-fold Rate Acceleration by Ethanol
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Date
2016-01-15
Authors
Zhou, Wenhu
Saran, Runjhun
Chen, Qingyun
Ding, Jinsong
Liu, Juewen
Journal Title
Journal ISSN
Volume Title
Publisher
Wiley
Abstract
Enzymes working in organic solvents are important for analytical chemistry, catalysis, and mechanistic studies. Although a few protein enzymes are highly active in organic solvents, little is known regarding nucleic acid-based enzymes. Herein, we report the first RNA-cleaving DNAzyme, named EtNa, that works optimally in concentrated organic solvents containing only monovalent Na+. The EtNa DNAzyme has a rate of 2.0 h(-1) in 54% ethanol (with 120 mm NaCl and no divalent metal ions), and a Kd of 21 mm Na+. It retains activity even in 72% ethanol as well as in DMSO. With 4 mm Na+, the rate in 54% ethanol is >1000-fold higher than that in water. We also demonstrated the use of EtNa to measuring the ethanol content in alcoholic drinks. In total, this DNAzyme has three unique features: divalent metal independent activity, Na+ selectivity among monovalent metals, and acceleration by organic solvents.
Description
This is the peer reviewed version of the following article: Zhou, W., Saran, R., Chen, Q., Ding, J., & Liu, J. (2016). A New Na+-Dependent RNA-Cleaving DNAzyme with over 1000-fold Rate Acceleration by Ethanol. Chembiochem, 17(2), 159–163, which has been published in final form at https://doi.org/10.1002/cbic.201500603. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Keywords
In-Vitro Selection, Alcoholic Beverages, Hammerhead Ribozyme, Nucleic-Acids, DNA Enzyme, Metal-Ions, Catalysis, Stability, Hairpin, Chromatography