dc.contributor.author | Zhou, Wenhu | |
dc.contributor.author | Ding, Jinsong | |
dc.contributor.author | Liu, Juewen | |
dc.date.accessioned | 2017-04-28 16:12:00 (GMT) | |
dc.date.available | 2017-04-28 16:12:00 (GMT) | |
dc.date.issued | 2016-09-20 | |
dc.identifier.uri | http://dx.doi.org/10.1093/nar/gkw845 | |
dc.identifier.uri | http://hdl.handle.net/10012/11798 | |
dc.description.abstract | Sodium is one of the most abundant metals in the environment and in biology, playing critical ecological and physiological roles. Na+ is also the most common buffer salt for nucleic acids research, while its specific interaction with DNA has yet to be fully studied. Herein, we probe a highly selective and robust Na+ aptamer using 2-aminopurine (2AP), a fluorescent adenine analog. This aptamer has two DNA strands derived from the Ce13d DNAzyme. By introducing a 2AP at the cleavage site of the substrate strand, Na+ induces similar to 40% fluorescence increase. The signaling is improved by a series of rational mutations, reaching > 600% with the C(10)A(20) double mutant. This fluorescence enhancement suggests relaxed base stacking near the 2AP label upon Na+ binding. By replacing a non-conserved adenine in the enzyme strand by 2AP, Na+-dependent fluorescence quenching is observed, suggesting that the enzyme loop folds into a more compact structure upon Na+ binding. The fluorescence changes allow for Na+ detection. With an optimized sequence, a detection limit of 0.4 mM Na+ is achieved, reaching saturated signal in less than 10 s. The sensor response is insensitive to ionic strength, which is critical for Na+ detection. | en |
dc.description.sponsorship | University of Waterloo, the Natural Sciences and Engineering Research Council of Canada (NSERC); Foundation for Shenghua Scholar of Central South University and the National Natural Science Foundation of China [21301195]; Fellowship from the China Scholarship Council (CSC) [201406370116 to W.Z.]. Funding for open access charge: Natural Sciences and Engineering Research Council of Canada (NSERC). | en |
dc.language.iso | en | en |
dc.publisher | Oxford University Press | en |
dc.rights | Attribution-NonCommercial 4.0 International | * |
dc.rights.uri | https://creativecommons.org/licenses/by-nc/4.0/ | * |
dc.subject | Lanthanide-Dependent Dnazyme | en |
dc.subject | In-Vitro Selection | en |
dc.subject | Resonance Energy-Transfer | en |
dc.subject | Uranyl-Specific Dnazyme | en |
dc.subject | RNA-Cleaving Dnazyme | en |
dc.subject | Metal-Ions | en |
dc.subject | Dna Enzyme | en |
dc.subject | Living Cells | en |
dc.subject | Sites | en |
dc.subject | Sensors | en |
dc.title | A highly specific sodium aptamer probed by 2-aminopurine for robust Na+ sensing | en |
dc.type | Article | en |
dcterms.bibliographicCitation | Zhou, W., Ding, J., & Liu, J. (2016). A highly specific sodium aptamer probed by 2-aminopurine for robust Na+ sensing. Nucleic Acids Research, 44(21), 10377–10385. https://doi.org/10.1093/nar/gkw845 | en |
uws.contributor.affiliation1 | Faculty of Science | en |
uws.contributor.affiliation2 | Chemistry | en |
uws.contributor.affiliation3 | Waterloo Institute for Nanotechnology (WIN) | en |
uws.typeOfResource | Text | en |
uws.peerReviewStatus | Reviewed | en |
uws.scholarLevel | Faculty | en |