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dc.contributor.authorHuang, Po-Jung Jimmy
dc.contributor.authorvan Ballegooie, Courtney
dc.contributor.authorLiu, Juewen 16:11:58 (GMT) 16:11:58 (GMT)
dc.description.abstractMercury is a highly toxic heavy metal and many DNA-based biosensors have been recently developed for Hg2+ detection in water. Among them, thymine-rich DNA is the most commonly used for designing Hg2+ sensors. However, the thymine-Hg2+ interaction is strongly affected by the buffer conditions. We recently reported a molecular beacon containing phosphorothioate (PS)-modified RNA linkages that can be cleaved by Hg2+. In this work, the fluorescence quenching and DNA adsorption properties of nano-sized graphene oxide (NGO) were used to develop a new sensor using the PS-RNA chemistry. Three DNA probes, containing one, three and five PS-RNA linkages, respectively, were tested. Finally, a fluorophore-labeled poly-A DNA with five PS-RNA linkages was selected and adsorbed by NGO. In the presence of Hg2+, the fluorophore was released from NGO due to the cleavage reaction, resulting in a fluorescence enhancement. This sensor is highly selective for Hg2+ with a detection limit of 8.5 nM Hg2+. For comparison, a fluorophore-labeled poly-T DNA was also tested, which responded to Hg2+ more slowly and was inhibited by high NaCl concentrations, while the PS-RNA probe was more tolerant to different buffer conditions. This work indicates a new method for interfacing DNA with NGO for Hg2+ detection.en
dc.description.sponsorshipNatural Sciences and Engineering Research Council of Canada (NSERC) [386326, STPGP 447472-13]en
dc.publisherRoyal Society of Chemistryen
dc.subjectColorimetric Detectionen
dc.subjectFluorescent Sensorsen
dc.subjectMercuric Ionen
dc.titleHg2+ detection using a phosphorothioate RNA probe adsorbed on graphene oxide and a comparison with thymine-rich DNAen
dcterms.bibliographicCitationHuang, P.-J. J., van Ballegooie, C., & Liu, J. (2016). Hg2+ detection using a phosphorothioate RNA probe adsorbed on graphene oxide and a comparison with thymine-rich DNA. Analyst, 141(12), 3788–3793.
uws.contributor.affiliation1Faculty of Scienceen
uws.contributor.affiliation3Waterloo Institute for Nanotechnology (WIN)en

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