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dc.contributor.authorBaeissa, Aifan
dc.contributor.authorDave, Neeshma
dc.contributor.authorSmith, Brendan D.
dc.contributor.authorLiu, Juewen 17:12:16 (GMT) 17:12:16 (GMT)
dc.descriptionThis document is the Accepted Manuscript version of a Published Work that appeared in final form in Applied Materials & Interfaces, copyright © American Chemical Society after peer review and technical editing by publisher. To access the final edited and published work see Baeissa, A., Dave, N., Smith, B. D., & Liu, J. (2010). DNA-Functionalized Monolithic Hydrogels and Gold Nanoparticles for Colorimetric DNA Detection. ACS Applied Materials & Interfaces, 2(12), 3594–3600.
dc.description.abstractHighly sensitive and selective DNA detection plays a central role in many fields of research, and various assay platforms have been developed. Compared to homogeneous DNA detection, surface-immobilized probes allow washing steps and signal amplification to give higher sensitivity. Previously research was focused on developing glass or gold-based surfaces for DNA immobilization; we herein report hydrogel-immobilized DNA. Specifically, acrydite-modified DNA was covalently functionalized to the polyacrylamide hydrogel during gel formation. There are several advantages of these DNA-functionalized monolithic hydrogels. First, they can be easily handled in a way similar to that in homogeneous assays. Second, they have a low optical background where, in combination with DNA-functionalized gold nanoparticles, even ∼0.1 nM target DNA can be visually detected. By using the attached gold nanoparticles to catalyze the reduction of Ag+, as low as 1 pM target DNA can be detected. The gels can be regenerated by a simple thermal treatment, and the regenerated gels perform similarly to freshly prepared ones. The amount of gold nanoparticles adsorbed through DNA hybridization decreases with increasing gel percentage. Other parameters including the DNA concentration, DNA sequence, ionic strength of the solution, and temperature have also been systematically characterized in this study.en
dc.description.sponsorshipUniversity of Waterloo || Natural Sciences and Engineering Research Council || Ministry of Higher Education of Saudi Arabia ||en
dc.publisherAmerican Chemical Societyen
dc.subjectColorimetric sensorsen
dc.titleDNA-Functionalized Monolithic Hydrogels and Gold Nanoparticles for Colorimetric DNA Detectionen
dcterms.bibliographicCitationBaeissa, A., Dave, N., Smith, B. D., & Liu, J. (2010). DNA-functionalized monolithic hydrogels and gold nanoparticles for colorimetric DNA detection. ACS applied materials & interfaces, 2(12), 3594-3600.en
uws.contributor.affiliation1Faculty of Scienceen
uws.contributor.affiliation2Waterloo Institute for Nanotechnology (WIN)en

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