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dc.contributor.authorHuang, Po-Jung Jimmy
dc.contributor.authorLiu, Juewen 15:28:20 (GMT) 15:28:20 (GMT)
dc.descriptionThis is the peer reviewed version of the following article: Huang, P.-J. J., & Liu, J. (2011). Immobilization of DNA on Magnetic Microparticles for Mercury Enrichment and Detection with Flow Cytometry. Chemistry - A European Journal, 17(18), 5004–5010, which has been published in final form at This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.en
dc.description.abstractMercury detection in water has attracted a lot of research interest due to its highly toxic nature and adverse environmental impact. In particular, the recent discovery of specific binding of HgII to thymine-rich (T-rich) DNA resulting in T-HgII-T base pairs has led to the development of a number of sensors with different signaling mechanisms. However, the majority of such sensors were non-immobilized. Immobilization, on the other hand, allows active mercury adsorption, signal amplification, and sensor regeneration. In this work, we immobilized a thymine-rich DNA on a magnetic microparticle (MMP) surface through biotin–streptavidin interactions. In the presence of HgII, the DNA changes from a random coil structure into a hairpin, upon which SYBR Green I binds to emit green fluorescence. Detection was carried out by using flow cytometry where the fluorescence intensity increased ≈9-fold in the presence of mercury and the binding of mercury reached equilibrium in less than 2 min. The sensor showed a unique sample-volume-dependent fluorescence signal change where a higher fluorescence was obtained with a larger sample volume, suggesting that the particles can actively adsorb HgII. Detection limits of 5 nM (1 ppb) and 14 nM (2.8 ppb) were achieved in pure buffer and in mercury-spiked Lake Ontario water samples, respectively.en
dc.description.sponsorshipUniversity of Waterloo || Natural Sciences and Engineering Research Council ||en
dc.subjectFlow cytometryen
dc.subjectMagnetic microparticlesen
dc.titleImmobilization of DNA on Magnetic Microparticles for Mercury Enrichment and Detection with Flow Cytometryen
dcterms.bibliographicCitationHuang, P.-J. J. and Liu, J. (2011), Immobilization of DNA on Magnetic Microparticles for Mercury Enrichment and Detection with Flow Cytometry. Chem. Eur. J., 17: 5004–5010. doi:10.1002/chem.201002934en
uws.contributor.affiliation1Faculty of Scienceen
uws.contributor.affiliation2Waterloo Institute for Nanotechnology (WIN)en

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