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dc.contributor.authorZhang, Xu
dc.contributor.authorServos, Mark R.
dc.contributor.authorLiu, Juewen 16:19:13 (GMT) 16:19:13 (GMT)
dc.descriptionThis document is the Accepted Manuscript version of a Published Work that appeared in final form in Langmuir copyright © American Chemical Society after peer review and technical editing by publisher. To access the final edited and published work see Zhang, X., Servos, M. R., & Liu, J. (2012). Surface Science of DNA Adsorption onto Citrate-Capped Gold Nanoparticles. Langmuir, 28(8), 3896–3902.
dc.description.abstractSingle-stranded DNA can be adsorbed by citrate capped gold nanoparticles (AuNPs), resulting in increased AuNP stability, which forms the basis of a number of biochemical and analytical applications, but the fundamental interaction of this adsorption reaction remains unclear. In this study, we measured DNA adsorption kinetics, capacity, and isotherms, demonstrating that the adsorption process is governed by electrostatic forces. The charge repulsion among DNA strands and between DNA and AuNPs can be reduced by adding salt, reducing pH or by using noncharged peptide nucleic acid (PNA). Langmuir adsorption isotherms are obtained, indicating the presence of both adsorption and desorption of DNA from AuNPs. While increasing salt concentration facilitates DNA adsorption, the desorption rate is also enhanced in higher salt due to DNA compaction. DNA adsorption capacity is determined by DNA oligomer length, DNA concentration, and salt. Previous studies indicated faster adsorption of short DNA oligomers by AuNPs, we find that once adsorbed, longer DNAs are much more effective in protecting AuNPs from aggregation. DNA adsorption is also facilitated by using low pH buffers and high alcohol concentrations. A model based on electrostatic repulsion on AuNPs is proposed to rationalize the DNA adsorption/desorption behavior.en
dc.description.sponsorshipUniversity of Waterloo || Canadian Foundation for Innovation || Ontario Ministry of Research & Innovation || Canadian Institutes of Health Research || Natural Sciences and Engineering Research Council ||en
dc.publisherAmerican Chemical Societyen
dc.subjectgold nanoparticlesen
dc.titleSurface Science of DNA Adsorption onto Citrate-Capped Gold Nanoparticlesen
dcterms.bibliographicCitationZhang, X., Servos, M. R., & Liu, J. (2012). Surface science of DNA adsorption onto citrate-capped gold nanoparticles. Langmuir, 28(8), 3896-3902.en
uws.contributor.affiliation1Faculty of Scienceen
uws.contributor.affiliation2Waterloo Institute for Nanotechnology (WIN)en

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