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dc.contributor.authorZhang, Xu
dc.contributor.authorLiu, Biwu
dc.contributor.authorServos, Mark R.
dc.contributor.authorLiu, Juewen
dc.date.accessioned2017-03-01 15:20:58 (GMT)
dc.date.available2017-03-01 15:20:58 (GMT)
dc.date.issued2013-05-21
dc.identifier.urihttp://dx.doi.org/10.1021/la400617u
dc.identifier.urihttp://hdl.handle.net/10012/11385
dc.descriptionThis document is the Accepted Manuscript version of a Published Work that appeared in final form in Langmuir, copyright © American Chemical Society after peer review and technical editing by publisher. To access the final edited and published work see Zhang, X., Liu, B., Servos, M. R., & Liu, J. (2013). Polarity Control for Nonthiolated DNA Adsorption onto Gold Nanoparticles. Langmuir, 29(20), 6091–6098. https://doi.org/10.1021/la400617uen
dc.description.abstractGold nanoparticles (AuNPs) functionalized with thiolated DNA have enabled many studies in nanoscience. The strong thiol/gold affinity and the nanoscale curvature of AuNPs allow the attached DNA to adapt an upright conformation favorable for hybridization. Recently, it has been shown that nonthiolated DNA can also be attached via DNA base adsorption. Without a thiol label, both ends of the DNA and even internal bases could be adsorbed, decreasing the specificity of subsequent molecular recognition reactions. In this work, we employed a modular sequence design approach to systematically study the effect of DNA sequence on adsorption polarity. A block of poly adenine (poly-A) could be used to achieve a high density of DNA attachment. When the poly-A block length is short (e.g., below 5–7), the loading was independent of the block length, and the conjugate cannot hybridize to its cDNA effectively, suggesting a random attachment controlled by adsorption kinetics. Increasing the block length leads to reduced capacity but improved hybridization, suggesting that more DNA with the desired conformation was adsorbed due to the thermodynamic effects of poly-A binding. The design can be further improved by including capping sequences rich in T or G. Finally, a more general double-stranded DNA approach was described to be suitable for DNA that cannot satisfy the above-mentioned design requirements.en
dc.description.sponsorshipUniversity of Waterloo || Canadian Institutes of Health Research || Canadian Foundation for Innovation || Natural Sciences and Engineering Research Council || Ontario Ministry of Research and Innovation ||en
dc.language.isoenen
dc.publisherAmerican Chemical Societyen
dc.subjectDNAen
dc.subjectgold nanoparticlesen
dc.subjectadsorptionen
dc.titlePolarity Control for Nonthiolated DNA Adsorption onto Gold Nanoparticlesen
dc.typeArticleen
dcterms.bibliographicCitationZhang, X., Liu, B., Servos, M. R., & Liu, J. (2013). Polarity Control for Nonthiolated DNA Adsorption onto Gold Nanoparticles. Langmuir, 29(20), 6091–6098. https://doi.org/10.1021/la400617uen
uws.contributor.affiliation1Faculty of Scienceen
uws.contributor.affiliation2Chemistryen
uws.contributor.affiliation2Waterloo Institute for Nanotechnology (WIN)en
uws.typeOfResourceTexten
uws.peerReviewStatusRevieweden
uws.scholarLevelFacultyen


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