dc.contributor.author | Liu, Zhenbao | |
dc.contributor.author | Chen, Shanshan | |
dc.contributor.author | Liu, Biwu | |
dc.contributor.author | Wu, Jianping | |
dc.contributor.author | Zhou, Yanbin | |
dc.contributor.author | He, Lingyun | |
dc.contributor.author | Ding, Jinsong | |
dc.contributor.author | Liu, Juewen | |
dc.date.accessioned | 2017-02-27 21:01:11 (GMT) | |
dc.date.available | 2017-02-27 21:01:11 (GMT) | |
dc.date.issued | 2014-12-16 | |
dc.identifier.uri | http://dx.doi.org/10.1021/ac503358m | |
dc.identifier.uri | http://hdl.handle.net/10012/11373 | |
dc.description | This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry copyright © American Chemical Society after peer review and technical editing by publisher. To access the final edited and published work see Liu, Z., Chen, S., Liu, B., Wu, J., Zhou, Y., He, L., … Liu, J. (2014). Intracellular Detection of ATP Using an Aptamer Beacon Covalently Linked to Graphene Oxide Resisting Nonspecific Probe Displacement. Analytical Chemistry, 86(24), 12229–12235. https://doi.org/10.1021/ac503358m | en |
dc.description.abstract | Fluorescent aptamer probes physisorbed on graphene oxide (GO) have recently emerged as a useful sensing platform. A signal is generated by analyte-induced probe desorption. To address nonspecific probe displacement and the false positive signal, we herein report a covalently linked aptamer probe for adenosine triphosphate (ATP) detection. A fluorophore and amino dual modified aptamer was linked to the carboxyl group on GO with a coupling efficiency of ∼50%. The linearity, specificity, stability, and regeneration of the covalent sensor were systematically studied and compared to the physisorbed probe. Both sensors have similar sensitivity, but the covalent one is more resistant to nonspecific probe displacement by proteins. The covalent sensor has a dynamic range from 0.125 to 2 mM ATP in buffer at room temperature and is resistance to DNase I. Intracellular ATP imaging was demonstrated using the covalent sensor, which generated a higher fluorescence signal than the physisorbed sensor. After the cells were stimulated with 5 mM Ca2+ for ATP production, the intracellular signal enhanced by 31.8%. This work highlights the advantages of covalent aptamer sensors using GO as both a quencher and a delivery vehicle for intracellular metabolite detection. | en |
dc.description.sponsorship | National Natural Science Foundation of China || Grant No. 81301258, 21301195
Hunan Provincial Natural Science Foundation of China || Grant No. 13JJ4029
Specialized Research Fund for the Doctoral Program of Higher Education of China || Grant No. 20130162120078
Postdoctoral Science Foundation of Central South University and China || Grant No. 124896
China Postdoctoral Science Foundation || Grant No. 2013M540644
International Postdoctoral Exchange Fellowship Program ||Grant No. 20140014
Shenghua Scholar Foundation ||
Natural Sciences and Engineering Research Council || | en |
dc.language.iso | en | en |
dc.publisher | American Chemical Society | en |
dc.subject | ATP | en |
dc.subject | graphene oxide | en |
dc.subject | Aptamer | en |
dc.title | Intracellular Detection of ATP Using an Aptamer Beacon Covalently Linked to Graphene Oxide Resisting Nonspecific Probe Displacement | en |
dc.type | Article | en |
dcterms.bibliographicCitation | Liu, Z., Chen, S., Liu, B., Wu, J., Zhou, Y., He, L., … Liu, J. (2014). Intracellular Detection of ATP Using an Aptamer Beacon Covalently Linked to Graphene Oxide Resisting Nonspecific Probe Displacement. Analytical Chemistry, 86(24), 12229–12235. https://doi.org/10.1021/ac503358m | en |
uws.contributor.affiliation1 | Faculty of Science | en |
uws.contributor.affiliation2 | Chemistry | en |
uws.contributor.affiliation2 | Waterloo Institute for Nanotechnology (WIN) | en |
uws.typeOfResource | Text | en |
uws.peerReviewStatus | Reviewed | en |
uws.scholarLevel | Faculty | en |