Comparative functional analysis of two alpha-glucosidases, Family 31 Glycoside Hydrolases from the human gut symbiont Bacteroides thetaiotaomicron

dc.contributor.authorChaudet, Marcia
dc.date.accessioned2012-04-18T18:45:31Z
dc.date.available2012-04-18T18:45:31Z
dc.date.issued2012-04-18T18:45:31Z
dc.date.submitted2012
dc.description.abstractThe human gut is home to a significant number of microorganisms including the dominant symbiont Bacteroides thetaiotaomicron. This microbe is predicted to possess an array of glycoside hydrolases, majority of which are involved in starch utilization. Presented here is a comparative functional analysis of two alpha-glucosidases, Family 31 Glycoside Hydrolases from Bacteroides thetaiotaomicron. Enzymatic kinetics revealed these enzymes both preferentially cleave alpha-(1,6) linkage in comparison to the predicted alpha-(1,4) and favour maltose derived substrates of longer length.en
dc.identifier.urihttp://hdl.handle.net/10012/6622
dc.language.isoenen
dc.pendingfalseen
dc.publisherUniversity of Waterlooen
dc.subjectBacteroides thetaiotaomicronen
dc.subjectalpha-glucosidaseen
dc.subject.programBiologyen
dc.titleComparative functional analysis of two alpha-glucosidases, Family 31 Glycoside Hydrolases from the human gut symbiont Bacteroides thetaiotaomicronen
dc.typeMaster Thesisen
uws-etd.degreeMaster of Scienceen
uws-etd.degree.departmentBiologyen
uws.peerReviewStatusUnrevieweden
uws.scholarLevelGraduateen
uws.typeOfResourceTexten

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