Application of solid phase microextraction for quantification of polyunstarurated fatty acids in biological fluids

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dc.contributor.authorPirjandi, Afsoon P.
dc.contributor.authorMirnaghi, Fatemeh Sadat
dc.contributor.authorBojko, Barbara
dc.contributor.authorWasowicz, Marcin
dc.contributor.authorPawliszyn, Janusz
dc.date.accessioned2016-03-01T19:40:54Z
dc.date.available2016-03-01T19:40:54Z
dc.date.issued2014
dc.description.abstractDevelopment of a straightforward strategy for simultaneous quantitative analysis of nonesterified fatty acids (NEFA) species in biofluids is a challenging task because of the extreme complexity of fatty acid distribution in biological matrices. In this study, we present a direct immersion solid phase microextraction method coupled to liquid chromatography-mass spectrometry platform (DI-SPME- HPLC-ESI -MS ) for determination of unconjugated fatty acids (FA) in fish and human plasma. The proposed method was fully validated according to bioanalytical method validation guidelines. The LOD and LOQ were in the range of 0.5-2 and 5-12 ng/ml, respec-tively, with a linear dynamic range of 100 fold for each compound. Absolute and relative matrix effects were comprehensively evaluated and found to be in the acceptable range of 91-116%. The affinity constant (Ka) of individual FAs to protein albumin was determined to be 9.2×104 to 4.3×105 M－1. The plasma protein binding (PPB%) was calculated, and found to be in the range of 98.0-99.7% for different polyunsaturated fatty acids (PUFAs). The PUFAs under study were found at a high concentration range in fish plasma, whereas only a few were within quantification range in control human plasma. The method was successfully applied for monitoring PUFA changes during the operation in plasma samples obtained from patients undergoing cardiac surgery with the use of cardiopulmonary bypass (CPB). The most significant contribution induced by surgery was noticed in the concentration level of α-linolenic acid (18:3, ALA), arachidonic acid (20:4, AA), and docosahexanoic acid (22:6, DHA) soon after administration of CPB in all cases.en
dc.description.sponsorshipNSERC Industrial Research Chair || NSERC IRCPJ184412-10 050165en
dc.identifier.urihttp://dx.doi.org/10.1021/ac502627w
dc.identifier.urihttp://hdl.handle.net/10012/10299
dc.language.isoenen
dc.publisherACSen
dc.relation.ispartofseriesAnalytical Chemistry;86, 12022-12029en
dc.titleApplication of solid phase microextraction for quantification of polyunstarurated fatty acids in biological fluidsen
dc.typeArticleen
dcterms.bibliographicCitationBirjandi A.P.; Mirnaghi F.S.; Bojko B.; Wasowicz M.; Pawliszyn J. Anal Chem 86, 12022-12029en
uws.contributor.affiliation1Faculty of Scienceen
uws.contributor.affiliation2Chemistryen
uws.peerReviewStatusRevieweden
uws.scholarLevelFacultyen
uws.typeOfResourceTexten

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