CONSTRUCTION AND OPTIMIZATION OF A ssDNA VECTOR PRODUCTION SYSTEM FOR GENE THERAPY PLATFORM
Loading...
Date
2023-06-15
Authors
PRAKASH, ROHINI
Advisor
Slavcev, Roderick
Journal Title
Journal ISSN
Volume Title
Publisher
University of Waterloo
Abstract
Viral capsid of Anellovirus and ssDNA reporter constructs carrying an anellovirus intergenic region were both produced separately in E. coli systems, purified and then mixed together in an in vitro system. At particular molar ratios we noted that the ssDNA was spontaneously encapsulated into anellovirus capsids. Viral vector structures were visualized by TEM and quantified by qPCR suggesting reasonable and scalable production efficiency.
Description
Keywords
Gene therapy, Anellovirus, Vector, Filamentous phage, ssDNA