Browsing Science (Faculty of) by Subject "aptamer"
Now showing items 1-5 of 5
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Biochemical Characterization of Fluorophore-Binding Nucleic Acids
(University of Waterloo, 2022-09-27)Aptamers, single stranded RNA or DNA that bind to target ligands with high affinity and specificity, have a wide range of applications, from therapeutics to biosensing, which makes biochemical characterization of the utmost ... -
Ligand Specificity and Affinity in the Sulforhodamine B Binding RNA Aptamer
(Elsevier Inc., 2020-07-18)Binding affinity and selectivity are critical properties of aptamers that must be optimized for any application. The sulforhodamine B binding RNA aptamer (SRB‐2) is a somewhat promiscuous aptamer that can bind ligands ... -
Selection and characterization of an aptamer to a thiazole orange derivative for use in a fluorescence-based biosensor
(University of Waterloo, 2023-08-18)Aptamers are short single-stranded DNA or RNA sequences that have been selected in vitro to have a high binding affinity to a specific target molecule. One class of aptamers can bind to small non-fluorescent molecules to ... -
Structure and Thermodynamics of Drug-RNA Aptamer Interactions
(Bentham Science Publishers, 2013-03-01)This mini-review will provide an overview on the recent studies of structure and thermodynamics of RNA aptamers that target drug molecules. These aptamers are studied to provide insight into RNA drug interactions. This ... -
Thioflavin T fluorescence and NMR spectroscopy 1 suggesting a non-G-quadruplex structure for a 2 sodium binding aptamer embedded in DNAzymes
(Canadian Science Publishing, 2021-09-16)Recently, a Na+-binding aptamer was reported to be embedded in a few RNA-cleaving DNAzymes including NaA43, Ce13d and NaH1. These DNAzymes require Na+ for activity but show no activity in the presence of K+ or other metal ...