Show simple item record

dc.contributor.authorSadatmousavi, Parisa
dc.date.accessioned2009-08-21 20:02:12 (GMT)
dc.date.available2009-08-21 20:02:12 (GMT)
dc.date.issued2009-08-21T20:02:12Z
dc.date.submitted2009-08-13
dc.identifier.urihttp://hdl.handle.net/10012/4577
dc.description.abstractAn ideal drug delivery system should contain an appropriate therapeutic agent and biocompatible carrier. In this study, we investigated the ability of the all-complementary self-assembling peptide AC8 in stabilizing the anticancer compound and determined the in-vitro therapeutic efficacy of the peptide-mediated anticancer drug delivery. The all-complementary peptide AC8 was designed based on the amino acid pairing principle (AAP), which contains hydrogen bonding, electrostatic, and hydrophobic interaction amino acid pairs. AAP interactions make the peptide capable of self-assembling into β-sheet structure in solution in a concentration dependent manner. Peptide solution concentration is a key parameter in controlling the nanoscale assembling of the peptide. The critical assembly concentration (CAC) of the peptide was found ~ 0.01 mg/ml by several techniques. The all-complementary peptide AC8 was found to be able to stabilize neutral state of hydrophobic anticancer compound ellipticine in aqueous solution. The formation of peptide-ellipticine complex was monitored by fluorescence spectroscopy at different mass ratios of peptide-to-ellipticine. The anticancer activity of the complexes with neutral state of ellipticine was found to show great anticancer activity against two cancer cells lines, A-549 and MCF-7. This peptide-mediated anticancer delivery system showed the induction of apoptosis on cancer cells in vitro by flow Cytometry.en
dc.language.isoenen
dc.publisherUniversity of Waterlooen
dc.subjectAll-complimentary self-assembling peptideen
dc.subjecthydrophobic anticancer drugen
dc.subjectdrug encapsulationen
dc.subjectcritical assembly concentrationen
dc.subjectCytotoxicityen
dc.subjectApoptosis inductionen
dc.titlePeptide-Mediated Anticancer Drug Deliveryen
dc.typeMaster Thesisen
dc.comment.hiddenPatent is still on process.en
dc.pendingfalseen
dc.subject.programChemical Engineeringen
uws-etd.degree.departmentChemical Engineeringen
uws-etd.degreeMaster of Applied Scienceen
uws.typeOfResourceTexten
uws.peerReviewStatusUnrevieweden
uws.scholarLevelGraduateen


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record


UWSpace

University of Waterloo Library
200 University Avenue West
Waterloo, Ontario, Canada N2L 3G1
519 888 4883

All items in UWSpace are protected by copyright, with all rights reserved.

DSpace software

Service outages