dc.contributor.author | Harfoot, Rhodri | |
dc.contributor.author | Yung, Deborah | |
dc.contributor.author | Anderson, William A. | |
dc.contributor.author | Wild, Cervantée E. K. | |
dc.contributor.author | Coetzee, Nicolene | |
dc.contributor.author | Hernández, Leonor C. | |
dc.contributor.author | Lawley, Blair | |
dc.contributor.author | Pletzer, Daniel | |
dc.contributor.author | Derraik, José G. B. | |
dc.contributor.author | Anderson, Yvonne | |
dc.contributor.author | Quiñones-Mateu, Miguel E. | |
dc.date.accessioned | 2022-06-09 19:56:50 (GMT) | |
dc.date.available | 2022-06-09 19:56:50 (GMT) | |
dc.date.issued | 2022-01-10 | |
dc.identifier.uri | https://doi.org/10.3390/pathogens11010083 | |
dc.identifier.uri | http://hdl.handle.net/10012/18366 | |
dc.description.abstract | The arrival of SARS-CoV-2 to Aotearoa/New Zealand in February 2020 triggered a massive response at multiple levels. Procurement and sustainability of medical supplies to hospitals and clinics during the then upcoming COVID-19 pandemic was one of the top priorities. Continuing access to new personal protective equipment (PPE) was not guaranteed; thus, disinfecting and reusing PPE was considered as a potential alternative. Here, we describe part of a local program intended to test and implement a system to disinfect PPE for potential reuse in New Zealand. We used filtering facepiece respirator (FFR) coupons inoculated with SARS-CoV-2 or clinically relevant multidrug-resistant pathogens (Acinetobacter baumannii Ab5075, methicillin-resistant Staphylococcus aureus USA300 LAC and cystic-fibrosis isolate Pseudomonas aeruginosa LESB58), to evaluate the potential use of ultraviolet-C germicidal irradiation (UV-C) or dry heat treatment to disinfect PPE. An applied UV-C dose of 1000 mJ/cm2 was sufficient to completely inactivate high doses of SARS-CoV-2; however, irregularities in the FFR coupons hindered the efficacy of UV-C to fully inactivate the virus, even at higher UV-C doses (2000 mJ/cm2). Conversely, incubating contaminated FFR coupons at 65 °C for 30 min or 70 °C for 15 min, was sufficient to block SARS-CoV-2 replication, even in the presence of mucin or a soil load (mimicking salivary or respiratory secretions, respectively). Dry heat (90 min at 75 °C to 80 °C) effectively killed 106 planktonic bacteria; however, even extending the incubation time up to two hours at 80 °C did not completely kill bacteria when grown in colony biofilms. Importantly, we also showed that FFR material can harbor replication-competent SARS-CoV-2 for up to 35 days at room temperature in the presence of a soil load. We are currently using these findings to optimize and establish a robust process for decontaminating, reusing, and reducing wastage of PPE in New Zealand. | en |
dc.description.sponsorship | UV Solutionz|| iDerm | en |
dc.language.iso | en | en |
dc.publisher | Multidisciplinary Digital Publishing Institute | en |
dc.rights | Attribution 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | SARS-CoV-2 | en |
dc.subject | COVID-19 | en |
dc.subject | personal protective equipment | en |
dc.subject | PPE | en |
dc.subject | disinfection | en |
dc.subject | bacteria | en |
dc.subject | UV-C | en |
dc.subject | New Zealand | en |
dc.title | Ultraviolet-C Irradiation, Heat, and Storage as Potential Methods of Inactivating SARS-CoV-2 and Bacterial Pathogens on Filtering Facepiece Respirators | en |
dc.type | Article | en |
dcterms.bibliographicCitation | Harfoot, R., Yung, D. B. Y., Anderson, W. A., Wild, C. E. K., Coetzee, N., Hernández, L. C., Lawley, B., Pletzer, D., Derraik, J. G. B., Anderson, Y. C., & Quiñones-Mateu, M. E. (2022). Ultraviolet-C Irradiation, Heat, and Storage as Potential Methods of Inactivating SARS-CoV-2 and Bacterial Pathogens on Filtering Facepiece Respirators. Pathogens, 11(1), 83. https://doi.org/10.3390/pathogens11010083 | en |
uws.contributor.affiliation1 | Faculty of Engineering | en |
uws.contributor.affiliation2 | Chemical Engineering | en |
uws.typeOfResource | Text | en |
uws.peerReviewStatus | Reviewed | en |
uws.scholarLevel | Faculty | en |