Show simple item record

dc.contributor.authorPan, Anni
dc.date.accessioned2022-05-02 12:56:50 (GMT)
dc.date.issued2022-05-02
dc.date.submitted2022-04-12
dc.identifier.urihttp://hdl.handle.net/10012/18211
dc.description.abstractUltrasound-induced cavitation of microbubbles has increased anticancer drug Doxorubicin (DOX) release from thermosensitive liposomes (TSL). Yet, the influence of liposomal formulations and their responses toward cavitation on controlled drug release of TSL is inadequately investigated. This novel research evaluates the contribution of heating and cavitation in enhancing drug release of biotinylated, doxorubicin-loaded thermosensitive liposomes (TSL-DOX) by changing liposomal formulations and ultrasound conditions. We have found that combining heating and cavitation triggers the maximum cumulative DOX release (80%~100%) than heating or cavitation only. The final cumulative drug release percentages are mainly dependent on the membrane compositions, which are influenced by changing the molar ratios of DSPE-PEG2K-biotin. Heating TSL-DOX to the phase transition temperature (Tm : ~ 42 ºC) is the driving force of DOX release (~ 70%). However, when the temperature is below Tm , cavitation can also trigger more DOX release of 10~20%. Cavitation (ultrasound frequency: 1 MHz, 10% duty cycle, 1kHz pulse repetition frequency, and 10 s exposure period) increased more DOX release by 20 ~ 25% after heating at 37 ºC and 42 ºC. Without cavitation, changing ultrasound parameters: burst cycles and exposure periods slightly influenced DOX release. Interestingly, increasing the molar ratios of DSPE-PEG2K-biotin decreased DOX release% even treating with cavitation and heating. To further evaluate the influence of cavitation on TSL-DOX, we also developed liposome-conjugated microbubbles (TSL-MB) via biotin-avidin-biotin linkage. DiO-labeled TSLDOX have been shown to conjugate to the surface of DiD-labeled microbubbles. After treating with heating and cavitation, TSL-MB improved DOX release within 5 min of heating, compared to TSLDOX. In summary, we successfully proved that combining cavitation and heating can improve drug release, and heating to T m is the major incentive to control drug release. These findings would support the development of novel liposomal drug control-release methods using both cavitation and heating.en
dc.language.isoenen
dc.publisherUniversity of Waterlooen
dc.subjectultrasound-induced cavitationen
dc.subjectmicrobubbleen
dc.subjectdoxorubicinen
dc.subjectthermosensitive liposomeen
dc.titleUltrasound-Induced Cavitation for Controlled Drug Release from Thermosensitive Liposomesen
dc.typeMaster Thesisen
dc.pendingfalse
uws-etd.degree.departmentElectrical and Computer Engineeringen
uws-etd.degree.disciplineElectrical and Computer Engineeringen
uws-etd.degree.grantorUniversity of Waterlooen
uws-etd.degreeMaster of Applied Scienceen
uws-etd.embargo.terms2 yearsen
uws.contributor.advisorYu, Alfred
uws.contributor.affiliation1Faculty of Engineeringen
uws.published.cityWaterlooen
uws.published.countryCanadaen
uws.published.provinceOntarioen
uws-etd.embargo2024-05-01T12:56:50Z
uws.typeOfResourceTexten
uws.peerReviewStatusUnrevieweden
uws.scholarLevelGraduateen


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record


UWSpace

University of Waterloo Library
200 University Avenue West
Waterloo, Ontario, Canada N2L 3G1
519 888 4883

All items in UWSpace are protected by copyright, with all rights reserved.

DSpace software

Service outages