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dc.contributor.authorMetherel, Adam Henry
dc.contributor.authorLacombe, R. J. Scott
dc.contributor.authorHenao, Aristizabal
dc.contributor.authorMorin-Rivron, Delphine
dc.contributor.authorKitson, Alex P.
dc.contributor.authorHopperton, Kathryn E.
dc.contributor.authorChalil, Daniel
dc.contributor.authorMasoodi, Mojgan
dc.contributor.authorStark, Ken
dc.contributor.authorBazinet, Richard P. 15:09:32 (GMT) 15:09:32 (GMT)
dc.descriptionThe final publication is available at Elsevier via © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
dc.description.abstractDocosahexaenoic acid (DHA, 22:6n-3) must be consumed in the diet or synthesized from n-3 polyunsaturated fatty acid (PUFA) precursors. However, the effect of dietary DHA on the metabolic pathway is not fully understood. Presently, 21-day-old Long Evans rats were weaned onto one of four dietary protocols: 1) 8 weeks of 2% ALA (ALA), 2) 6 weeks ALA followed by 2 weeks of 2% ALA + 2% DHA (DHA), 3) 4 weeks ALA followed by 4 weeks DHA and 4) 8 weeks of DHA. After the feeding period, 2H5-ALA and 13C20-eicosapentaenoic acid (EPA, 20:5n-3) were co-infused and blood was collected over 3 h for determination of whole-body synthesis-secretion kinetics. The synthesis-secretion coefficient (ml/min, means ± SEM) for EPA (0.238±0.104 vs. 0.021±0.001) and DPAn-3 (0.194±0.060 vs. 0.020±0.008) synthesis from plasma unesterified ALA, and DPAn-3 from plasma unesterified EPA (2.04±0.89 vs. 0.163±0.025) were higher (P<.05) after 2 weeks compared to 8 weeks of DHA feeding. The daily synthesis-secretion rate (nmol/d) of DHA from EPA was highest after 4 weeks of DHA feeding (843±409) compared to no DHA (70±22). Liver gene expression of ELOVL2 and FADS2 were lower (P<.05) after 4 vs. 8 weeks of DHA. Higher synthesis-secretion kinetics after 2 and 4 weeks of DHA feeding suggests an increased throughput of the PUFA metabolic pathway. Furthermore, these findings may lead to novel dietary strategies to maximize DHA levels while minimizing dietary requirements.en
dc.description.sponsorshipNatural Sciences and Engineering Research Council of Canada || 482597en
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.subjectdocosahexaenoic aciden
dc.subjectN-3 fatty acidsen
dc.titleTwo weeks of docosahexaenoic acid (DHA) supplementation increases synthesis-secretion kinetics of n-3 polyunsaturated fatty acids compared to 8 weeks of DHA supplementationen
dcterms.bibliographicCitationMetherel, A. H., Lacombe, R. J. S., Aristizabal Henao, J. J., Morin-Rivron, D., Kitson, A. P., Hopperton, K. E., … Bazinet, R. P. (2018). Two weeks of docosahexaenoic acid (DHA) supplementation increases synthesis-secretion kinetics of n-3 polyunsaturated fatty acids compared to 8 weeks of DHA supplementation. The Journal of Nutritional Biochemistry, 60, 24–34.
uws.contributor.affiliation1Faculty of Applied Health Sciencesen

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