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dc.contributor.authorZhou, Wenhu
dc.contributor.authorDing, Jinsong
dc.contributor.authorLiu, Juewen 16:12:03 (GMT) 16:12:03 (GMT)
dc.descriptionThe final publication is available at Elsevier via © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
dc.description.abstractHeavy metal detection is a key topic in analytical chemistry. DNA-based metal recognition has advanced significantly producing many specific metal ligands, such as thymine for Hg2+ and cytosine for Ag+. For practical applications, however, robust sensors that can work in a diverse range of salt concentrations need to be developed, while most current sensing strategies cannot meet this requirement. In this work, 2-aminopurine (2AP) is used as a fluorescence label embedded in the middle of four 10-mer DNA homopolymers. 2AP can be quenched up to 98% in these DNA without an external quencher. The interaction between 2AP and all common metal ions is studied systematically for both free 2AP base and 2AP embedded DNA homopolymers. With such low background, Hg2+ induces up to 14-fold signal enhancement for the poly-T DNA, and Ag+ enhances up to 10-fold for the poly-C DNA. A detection limit of 3 nM is achieved for both metals. With these four probes, silver and mercury can be readily discriminated from the rest. A comparison with other signaling methods was made using fluorescence resonance energy transfer, graphene oxide, and SYBR Green I staining, respectively, confirming the robustness of the 2AP label. Detection of Hg2+ in Lake Huron water was also achieved with a similar sensitivity. This work has provided a comprehensive fundamental understanding of using 2AP as a label for metal detection, and has achieved the highest fluorescence enhancement for non-protein targets. (C) 2016 Elsevier B.V. All rights reserved.en
dc.description.sponsorshipUniversity of Waterloo; Natural Sciences and Engineering Research Council of Canada (NSERC) [386326]; Foundation for Shenghua Scholar of Central South University; National Natural Science Foundation of China [21301195]; China Scholarship Council (CSC) [201406370116]en
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.subjectMolecular Beaconsen
dc.subjectSelective Detectionen
dc.subjectSignaling Aptamersen
dc.title2-Aminopurine-modified DNA homopolymers for robust and sensitive detection of mercury and silveren
dcterms.bibliographicCitationZhou, W., Ding, J., & Liu, J. (2017). 2-Aminopurine-modified DNA homopolymers for robust and sensitive detection of mercury and silver. Biosensors & Bioelectronics, 87, 171–177.
uws.contributor.affiliation1Faculty of Scienceen
uws.contributor.affiliation3Waterloo Institute for Nanotechnology (WIN)en

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