dc.contributor.author | Jacobi, Zachary E. | |
dc.contributor.author | Li, Lu | |
dc.contributor.author | Liu, Juewen | |
dc.date.accessioned | 2017-03-01 18:26:23 (GMT) | |
dc.date.available | 2017-03-01 18:26:23 (GMT) | |
dc.date.issued | 2012-02-07 | |
dc.identifier.uri | http://dx.doi.org/10.1039/C2AN15754C | |
dc.identifier.uri | http://hdl.handle.net/10012/11401 | |
dc.description.abstract | Lead is highly toxic and its detection has attracted a lot of research interests. In recent years, DNA has been used for Pb2+ recognition and many fluorescent sensors with low to sub-nM detection limits have been reported. These figures of merit were typically measured using a spectrophotometer that can detect nM DNA with a high signal-to-noise ratio. For visual detection, however, μM DNA or dye was required, making it difficult to detect low nM targets. We recently achieved a visual sensitivity of 10 nM Hg2+ by immobilizing a DNA probe in a hydrogel. This was made possible because the gel was able to actively adsorb Hg2+. In this work, we aim to test whether this method can be extended to the detection of Pb2+. First, a new Pb2+ sensor was designed based on a guanine-rich DNA and DNA binding dyes such as thiazole orange and SYBR Green I. The free DNA showed a detection limit of 8 nM Pb2+ using 40 nM DNA. For visual detection in solution with 1 μM of the DNA probe, however, ∼300 nM Pb2+ was required. After immobilization in a monolithic polyacrylamide hydrogel, even 20 nM Pb2+ could be visually detected with a sample volume of 50 mL. Therefore, sensitive detection without signal amplification was achieved. Finally, we demonstrated simultaneous detection of both Hg2+ and Pb2+ in the same water sample with shape encoded hydrogel sensors. | en |
dc.description.sponsorship | University of Waterloo ||
Canada Foundation for Innovation ||
Natural Sciences and Engineering Research Council ||
Ontario Ministry of Research and Innovation || | en |
dc.language.iso | en | en |
dc.publisher | Royal Society of Chemistry | en |
dc.subject | lead | en |
dc.subject | DNA | en |
dc.subject | hydrogel | en |
dc.title | Visual detection of lead(II) using a label-free DNA-based sensor and its immobilization within a monolithic hydrogel | en |
dc.type | Article | en |
dcterms.bibliographicCitation | Jacobi, Z. E., Li, L., & Liu, J. (2012). Visual detection of lead (II) using a label-free DNA-based sensor and its immobilization within a monolithic hydrogel. Analyst, 137(3), 704-709. | en |
uws.contributor.affiliation1 | Faculty of Science | en |
uws.contributor.affiliation2 | Chemistry | en |
uws.contributor.affiliation2 | Waterloo Institute for Nanotechnology (WIN) | en |
uws.typeOfResource | Text | en |
uws.peerReviewStatus | Reviewed | en |
uws.scholarLevel | Faculty | en |