UWSpace will be migrating to a new version of its software from July 29th to August 1st. UWSpace will be offline for all UW community members during this time.

Show simple item record

dc.contributor.authorGómez-Ríos, Germán Augusto
dc.contributor.authorReyes-Garcés, Nathaly
dc.contributor.authorBojko, Barbara
dc.contributor.authorPawliszyn, Janusz
dc.date.accessioned2016-11-08 20:56:50 (GMT)
dc.date.available2016-11-08 20:56:50 (GMT)
dc.date.issued2015-12-09
dc.identifier.urihttp://dx.doi.org/10.1021/acs.analchem.5b03668
dc.identifier.urihttp://hdl.handle.net/10012/11068
dc.descriptionThis document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://pubs.acs.org/doi/abs/10.1021/acs.analchem.5b03668en
dc.description.abstractIn recent years, different geometrical configurations of solid-phase microextraction (SPME) have been directly coupled to mass spectrometry, resulting in benefits such as diminishing matrix effects, improvement of detection limits, and considerable enhancement of analysis throughput. Although SPME fibers have been used for years, their potential for quantitative analysis when directly combined with mass spectrometry has not been explored to its full extent. In this study, we present the direct coupling of biocompatible SPME (Bio-SPME) fibers to mass spectrometry via nanoelectrospray ionization (nano-ESI) emitters as a powerful tool for fast quantitative analysis of target analytes in biofluids. Total sample preparation time does not exceed 2 min, and by selecting an appropriate fiber length and sample vessel, sample volumes ranging between 10 and 1500 μL can be used. Despite the short extraction time of the technique, limits of detection in the subnanogram per milliliter with good accuracy (≥90%) and linearity (R2 > 0.999) were attained for all the studied probes in phosphate-buffered saline (PBS), urine, and whole blood. Given that Bio-SPME–nano-ESI efficiently integrates sampling with analyte extraction/enrichment, sample cleanup (including elimination of matrix effects in the form of particles), and ionization, our results demonstrated that it is an advantageous configuration for bioanalytical applications such as therapeutic drug monitoring, doping in sports, and pharmacological studies in various matrixes.en
dc.description.sponsorshipthe Natural Sciences and Engineering Research Council (NSERC) of Canadaen
dc.language.isoenen
dc.publisherAmerican Chemical Societyen
dc.titleBiocompatible Solid-Phase Microextraction Nanoelectrospray Ionization: An Unexploited Tool in Bioanalysisen
dc.typeArticleen
dcterms.bibliographicCitationGómez-Ríos, G. A., Reyes-Garcés, N., Bojko, B., & Pawliszyn, J. (2016). Biocompatible Solid-Phase Microextraction Nanoelectrospray Ionization: An Unexploited Tool in Bioanalysis. Analytical Chemistry, 88(2), 1259–1265. https://doi.org/10.1021/acs.analchem.5b03668en
uws.contributor.affiliation1Faculty of Scienceen
uws.contributor.affiliation2Chemistryen
uws.typeOfResourceTexten
uws.peerReviewStatusRevieweden
uws.scholarLevelFacultyen


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record


UWSpace

University of Waterloo Library
200 University Avenue West
Waterloo, Ontario, Canada N2L 3G1
519 888 4883

All items in UWSpace are protected by copyright, with all rights reserved.

DSpace software

Service outages