Browsing Waterloo Research by Author "Dieckmann, Thorsten"
Now showing items 1-13 of 13
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Calmodulin regulates Cav3 T-type channels at their gating brake
Chemin, Jean; Taiakina, Valentina; Monteil, Arnaud; Piazza, Michael; Guan, Wendy; Stephens, Robert F.; Kitmitto, Ashraf; Pang, Zhiping P.; Dolphin, Annette C.; Perez-Reyes, Edward; Dieckmann, Thorsten; Guillemette, J. Guy; Spafford, J. David (American Society for Biochemistry and Molecular Biology, 2017-12-08)Calcium (Cav1 and Cav2) and sodium channels possess homologous CaM-binding motifs, known as IQ motifs in their C termini, which associate with calmodulin (CaM), a universal calcium sensor. Cav3 T-type channels, which serve ... -
Chemical shift assignments of calmodulin constructs with EF hand mutations
Piazza, Michael; Guillemette, J. Guy; Dieckmann, Thorsten (Springer, 2016-04-01)Calmodulin (CaM) is a ubiquitous cytosolic Ca2+-binding protein able to bind and regulate hundreds of different proteins. It consists of two globular domains joined by a flexible central linker region. Each one of these ... -
Chemical shift perturbations induced by residue specific mutations of CaM interacting with NOS peptides
Piazza, Michael; Guillemette, J. Guy; Dieckmann, Thorsten (Springer, 2015-10-01)The regulation of nitric oxide synthase (NOS) by calmodulin (CaM) plays a major role in a number of key physiological and pathological processes. A detailed molecular level picture of how this regulation is achieved is ... -
Dynamics of Nitric Oxide Synthase–Calmodulin Interactions at Physiological Calcium Concentrations
Piazza, Michael; Guillemette, J. Guy; Dieckmann, Thorsten (American Chemical Society, 2015-03-24)The intracellular Ca2+ concentration is an important regulator of many cellular functions. The small acidic protein calmodulin (CaM) serves as a Ca2+ sensor and control element for many enzymes. Nitric oxide synthase (NOS) ... -
Identifying Fenton-Reacted Trimethoprim Transformation Products Using Differential Mobility Spectrometry
Psutka, Jarrod; Dion-Fortier, Annick; Dieckmann, Thorsten; Campbell, J. Larry; Segura, Pedro; Hopkins, W. Scott (ACS Publications, 2018-04-17)A transformation product of trimethoprim, a contaminant of emerging concern in the environment, is generated using an electro-assisted Fenton reaction and analyzed using differential mobility spectrometry (DMS) in combination ... -
Ligand Specificity and Affinity in the Sulforhodamine B Binding RNA Aptamer
Piccolo, Kyle; McNeil, Brooke; Crouse, Jeff; Lim, Su Ji; Hopkins, W. Scott; Dieckmann, Thorsten (Elsevier Inc., 2020-07-18)Binding affinity and selectivity are critical properties of aptamers that must be optimized for any application. The sulforhodamine B binding RNA aptamer (SRB‐2) is a somewhat promiscuous aptamer that can bind ligands ... -
Separating and probing tautomers of protonated nucleobases using differential mobility spectrometry
Anwar, Ahdia; Psutka, Jarrod; Walker, Stephen W. C.; Dieckmann, Thorsten; Janizewski, John S.; Larry Campbell, J.; Scott Hopkins, W. (Elsevier, 2017-08-26)The protonated nucleobases (C+H)+, (T+H)+, (U+H)+, (A+H)+, and (G+H)+ are investigated in a combined experimental and computational study using differential mobility spectrometry (DMS), mass spectrometry, and electronic ... -
Solution Structure of Calmodulin Bound to the Target Peptide of Endothelial Nitric Oxide Synthase Phosphorylated at Thr495
Piazza, Michael; Taiakina, Valentina; Guillemette, Simon R.; Guillemette, J. Guy; Dieckmann, Thorsten (American Chemical Society, 2014-03-04)Nitric oxide synthase (NOS) plays a major role in a number of key physiological and pathological processes, and it is important to understand how this enzyme is regulated. The small acidic calcium binding protein, calmodulin ... -
Structural Consequences of Calmodulin EF Hand Mutations
Piazza, Michael; Taiakina, Valentina; Dieckmann, Thorsten; Guillemette, J. Guy (American Chemical Society, 2017-02-21)Calmodulin (CaM) is a cytosolic Ca2+-binding protein that serves as a control element for many enzymes. It consists of two globular domains, each containing two EF hand pairs capable of binding Ca2+, joined by a flexible ... -
Structural Studies of a Complex Between Endothelial Nitric Oxide Synthase and Calmodulin at Physiological Calcium Concentration
Piazza, Michael; Dieckmann, Thorsten; Guillemette, J. Guy (American Chemical Society, 2016-10-25)The small acidic protein calmodulin (CaM) serves as a Ca2+ sensor and control element for many enzymes including nitric oxide synthase (NOS) enzymes that play major roles in key physiological and pathological processes. ... -
Structure and Thermodynamics of Drug-RNA Aptamer Interactions
Da Costa, Jason B.; Dieckmann, Thorsten (Bentham Science Publishers, 2013-03-01)This mini-review will provide an overview on the recent studies of structure and thermodynamics of RNA aptamers that target drug molecules. These aptamers are studied to provide insight into RNA drug interactions. This ... -
Thermodynamics and Kinetics of Adaptive Binding in the Malachite Green RNA Aptamer
Da Costa, Jason B.; Andreiev, Aurelia I.; Dieckmann, Thorsten (American Chemical Society, 2013-09-24)Adaptive binding, the ability of molecules to fold themselves around the structure of a ligand and thereby incorporating it into their three-dimensional fold, is a key feature of most RNA aptamers. The malachite green ... -
Thioflavin T fluorescence and NMR spectroscopy 1 suggesting a non-G-quadruplex structure for a 2 sodium binding aptamer embedded in DNAzymes
Saran, Runjhun; Piccolo, Kyle A.; He, Yanping; Kang, Yongqiang; Huang, Po-Jung Jimmy; Wei, Chunying; Chen, Da; Dieckmann, Thorsten; Liu, Juewen (Canadian Science Publishing, 2021-09-16)Recently, a Na+-binding aptamer was reported to be embedded in a few RNA-cleaving DNAzymes including NaA43, Ce13d and NaH1. These DNAzymes require Na+ for activity but show no activity in the presence of K+ or other metal ...