Browsing Science (Faculty of) by Supervisor "Liu, Juewen"
Now showing items 1-17 of 17
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Aptamer-Based Detection of Caffeine and Theophylline Through Utilization of Gold Nanoparticles and Graphene Oxide
(University of Waterloo, 2023-04-26)The caffeine and theophylline aptamers were used in tandem with gold nanoparticles and graphene oxide to study potential methods for small molecule detection. The AuNP approach is a label-free approach, and the graphene ... -
Binding of Au3+ Ions by Polyadenine DNA
(University of Waterloo, 2020-09-02)Interactions between DNA and metal ions are important for many applications such as metal sensing, therapeutics and nanotechnology. Many studies have already been published on these topics. DNA can bind metal ions via its ... -
DNA and Hydrogels for Sensing and Binding Purine Nucleosides
(University of Waterloo, 2021-08-27)Purine nucleosides, such as adenosine and guanosine, are important biomolecules to regulate physiological functions, including maintain heart and brain health, exert inflammatory responses, and take part in metabolisms. ... -
DNA and Metal Ion Mediated Modification of Nanomaterials
(University of Waterloo, 2021-09-01)DNA-modified nanomaterials have been applied in diverse areas such as biosensing, catalysis, drug delivery, and biomedical diagnostics. Metal ion mediated DNA conjugation is an important strategy for the construction of ... -
DNA/Metal Oxide Nanoconjugates: Fundamental Understandings and Analytical Applications
(University of Waterloo, 2016-07-29)DNA-functionalized nanomaterials have shown versatile applications in biosensor development, biomedical diagnostics, therapy, and catalysis. DNA is attractive for this purpose for its programmable structure, molecular ... -
A Highly Active RNA-Cleaving DNAzyme with Accelerated Activity by Ethanol and by Freezing
(University of Waterloo, 2018-04-27)DNAzymes are functional DNA molecules with catalytic activities. The EtNa is an example of a RNA-cleaving DNAzyme that catalyzes the cleavage reaction of a phosphodiester bond in the presence of metal ions, such as Na+ or ... -
In vitro selection and biochemical characterization of trivalent metal ion dependent RNA-cleaving DNAzymes
(University of Waterloo, 2016-08-30)DNAzymes are DNA sequences with catalytic activity. So far, all known DNAzymes have been isolated using the high-throughput in vitro selection method. DNAzymes have been used for analytical, biomedical, and nanotechnology ... -
In Vitro Selection, Characterization, and Application of a Sodium-Dependent DNAzyme and a Metal-Independent Ribozyme
(University of Waterloo, 2021-09-20)Ribozymes and DNAzymes are collectively called catalytic nucleic acids. Compared to protein enzymes, catalytic nucleic acids are programmable in structure, easy to synthesize or modify, and more chemically stable especially ... -
In-vitro selection, characterization and application of a silver-specific DNAzyme
(University of Waterloo, 2019-01-07)Evolution of novel DNA-based catalysts (DNAzymes) through in-vitro selection has expedited the research by manifolds, in both fundamental and analytical aspects of bio-nanotechnology. DNAzymes are attractive for their high ... -
Interfacing fluorescent DNA oligonucleotides with graphene oxide and metal oxides: from adsorption to sensing
(University of Waterloo, 2022-08-18)DNA, apart from being the mode of genomic information storage, has found several uses in catalysis (DNAzymes) and target detection (aptamers). Developing novel biosensors utilizing these properties has therefore been a ... -
Interfacing Liposomes with Metal Ions and Metal Oxides: Coordination Chemistry, Surface Forces and Membrane Integrity
(University of Waterloo, 2020-09-04)Liposomes are self-closed vesicles composed of lipid bilayer walls that have been widely used as models for cell membranes and drug delivery vehicles. One interesting research direction is to study the interactions of ... -
Investigation of divalent metal ion detection using 17E DNAzyme Biosensor
(University of Waterloo, 2021-04-30)DNAzymes are in vitro selected oligonucleotides that can catalyze reactions, such as RNA cleavage. Most DNAzymes require metal ions as cofactors. Unlike ribozymes, which have been found in nature, all known DNAzymes are ... -
Metal-mediated Adsorption of DNA Oligonucleotides onto Polymeric Materials
(University of Waterloo, 2022-12-20)In recent decades, DNA oligonucleotides have been extensively researched due to their applications for catalysis (DNAzymes), biosensing (DNA aptamers/Watson-Crick hybridization), and nanomaterials assembly (DNA origami). ... -
Molecular Imprinting with Functional DNA and Nanozymes: Affinity Improvement and Selective Catalysis
(University of Waterloo, 2019-08-19)Molecular imprinting refers to polymerization of functional monomers in the presence of a template molecule. It is a general method to prepare stable and cost-effective artificial ligands as antibody mimics (also known as ... -
A New Na(+)-specific DNAzyme Mutant from in Vitro Selection
(University of Waterloo, 2017-09-08)Sodium is one of the most ubiquitous metal ions in both intracellular and extracellular fluids. Many fluorescent sensors have been designed to measure Na+ concentrations. However, Na+ binding to biomolecules such as DNA ... -
Parallel Polyadenine Duplex Formation at Low pH Facilitates DNA Conjugation onto Gold Nanoparticles
(University of Waterloo, 2017-04-20)DNA-functionalized gold nanoparticles (AuNPs) have been extensively used in sensing, drug delivery, and materials science. A key step is to attach DNA onto AuNPs forming a stable and functional conjugate. While the traditional ... -
Synthesis of Phosphate-Crosslinked Starch Nanoparticles for Drug Delivery
(University of Waterloo, 2017-04-25)Drug delivery agents for chemotherapy drugs have gained significant interest over past few decades due to the need to localize the treatment to cancer cells. So far, polymeric micelles, liposomes, and carbon-based ...