Browsing Science (Faculty of) by Subject "in vitro"
Now showing items 1-10 of 10
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Antifungal ocular drug delivery via contact lenses using a novel in vitro eye model
(University of Waterloo, 2016-04-21)Purpose: The purpose of this thesis was to evaluate the potential of contact lenses (CLs) as an antifungal drug delivery device, and to develop an in vitro eye model to test thereof. Methods: The first three chapters ... -
The characterization of γH2AX as a biomarker of genotoxicity in Oncorhynchus mykiss
(University of Waterloo, 2017-05-19)Environmental contamination due to by-products and waste from industrial activities is a growing universal concern, especially regarding the impact on water and the aquaculture industry. Conventional tests to diagnose ... -
The combined effects of benzalkonium chloride and ultraviolet radiation on the bovine lens in vitro
(University of Waterloo, 2018-04-23)Purpose: To determine the combined effects of benzalkonium chloride (BAK) and ultraviolet radiation (UV) using the primary culture of intact bovine lenses. Methods: Abattoir-provided lenses were dissected from the eyes ... -
Development of an in vitro eye model to better represent in vivo physiological conditions
(University of Waterloo, 2021-06-23)Purpose The purpose of this thesis was to optimize a novel in vitro blink model, the OcuBlink, to best mimic the physiological characteristics of the human eye. By improving this in vitro model, the results from in vitro ... -
Development of RTgutGC as a Tool for Fish Feed Development
(University of Waterloo, 2017-08-15)A rainbow trout intestinal epithelial cell line, RTgutGC, has been used in this thesis to investigate the effects of various stresses in aquaculture on the gastrointestinal (GI) tract and for identifying possible beneficial ... -
Development of Sensitive In Vitro Assays to Assess the Ocular Toxicity Potential of Chemicals and Ophthalmic Products
(University of Waterloo, 2009-04-27)The utilization of in vitro tests with a tiered testing strategy for detection of mild ocular irritants can reduce the use of animals for testing, provide mechanistic data on toxic effects, and reduce the uncertainty ... -
Metabolomic Profiling of Nutrient Solutions for Characterization of Human Gut Microbiota
(University of Waterloo, 2015-01-06)Studying the metabolite output, the “exometabolome,” of bacterial communities provides insight into the metabolic interactions occurring within that ecosystem. As such, a metabolomic approach can be used to gain further ... -
Structure and Thermodynamics of Drug-RNA Aptamer Interactions
(Bentham Science Publishers, 2013-03-01)This mini-review will provide an overview on the recent studies of structure and thermodynamics of RNA aptamers that target drug molecules. These aptamers are studied to provide insight into RNA drug interactions. This ... -
Toxicity of Ophthalmic Solutions, Benzalkonium Chloride and UV Radiation on Human Corneal Epithelial Cells in vitro
(University of Waterloo, 2015-06-12)The purpose of this work was to develop a sensitive in vitro method for ocular toxicity testing; and to use this method to assess the individual and combined effects of ophthalmic solutions, benzalkonium chloride (BAK), ... -
Use of Fish Cell Cultures for the Study and Cultivation of Microsporidia
(University of Waterloo, 2011-08-26)Microsporidia are a group of obligate intracellular fungal parasites that infect a wide range of vertebrates and invertebrates, and are of economic and academic interest. Some areas of their economic impact are in aquaculture ...