Browsing University of Waterloo by Supervisor "Holyoak, Todd"
Now showing items 1-6 of 6
-
Establishing Viable Systems for the Recombinant Production of Haemophilus influenzae Immunoglobulin A1 Protease
(University of Waterloo, 2020-08-20)Immunoglobulin A1 proteases (IgAPs) are a family of enzymatic virulence factors secreted from a wide variety of pathogenic bacteria known to infect human mucosal surfaces. Once secreted, IgAPs cleave and inactivate the ... -
Functional consequences of changing structure, dynamics, and free-energy landscapes of phosphoenolpyruvate carboxykinases
(University of Waterloo, 2020-09-21)The complex relationship between structure, dynamics, and function will afford insights into the operational details of enzymes. To understand, regulate, and create enzymes, this complex nature must be comprehensively ... -
The HOTHEAD Protein: Assessing Enzymatic Activity using Computational and Recombinant Protein Expression Approaches
(University of Waterloo, 2019-04-30)In Arabidopsis thaliana, a number of genes regulating cuticle synthesis have been identified by virtue of organ fusion phenotype. One such gene, HOTHEAD (HTH) was among those originally identified by this phenotype but its ... -
Influence of Temperature on the Catalytic Function of Phosphoenolpyruvate Carboxykinase
(University of Waterloo, 2019-05-22)Enzymes are well known for their importance in biological systems. One of these enzymes, PEPCK, has been well studied and characterized over the years. Successful catalysis in PEPCK is very dependent on mobile loop elements ... -
Inhibition of Phosphoenolpyruvate Carboxykinase with a 3-Mercaptopicolinic acid Analogue
(University of Waterloo, 2016-09-01)Phosphoenolpyruvate carboxykinase (PEPCK) is the metabolic enzyme that catalyzes the first committed step in gluconeogenesis and glyceroneogensis. More recently, PEPCK has been implicated in the maintenance and establishment ... -
Structural and kinetic characterization of the mechanism of regulation of phosphoenolpyruvate carboxykinase by anions
(University of Waterloo, 2019-12-19)Phosphoenolpyruvate carboxykinase (PEPCK) catalyses a reaction that is known to be thermodynamically reversible. In contrast to this thermodynamic reversibility, in eukaryotes, PEPCK is observed to primarily catalyze the ...