Browsing University of Waterloo by Subject "Metabolomics"
Now showing items 1-7 of 7
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A correction method for systematic error in 1H-NMR time-course data validated through stochastic cell culture simulation
(BioMed Central, 2015-09-04)Background: The growing ubiquity of metabolomic techniques has facilitated high frequency time-course data collection for an increasing number of applications. While the concentration trends of individual metabolites can ... -
Coupling solid phase microextraction to complementary separation platforms for metabotyping of E. coli metabolome in response to natural antibacterial agents
(Springer, 2016-11-02)Introduction Essential oils are known to possess antimicrobial activity; thus, their use has played an important role over the years in medicine and for food preservation purposes. Objective The effect of clove oil and its ... -
Developments and applications of solid phase microextraction (SPME) coupled to direct analysis in real time (DART) and approaches towards small volume analysis
(University of Waterloo, 2019-08-21)Solid phase microextraction (SPME) has been rapidly developing since its invention in 1990. Due to a flexible design and the ability to implement various extractive coatings, SPME has found use in numerous applications. ... -
From Metabolite Concentration to Flux – A Systematic Assessment of Error in Cell Culture Metabolomics
(University of Waterloo, 2016-08-05)The growing availability of genomic, transcriptomic, and metabolomic data has opened the door to the synthesis of multiple levels of information in biological research. As a consequence, there has been a push to analyze ... -
In vivo microsampling to capture the elusive exposome
(Nature Publishing Group, 2017-03-07)Loss and/or degradation of small molecules during sampling, sample transportation and storage can adversely impact biological interpretation of metabolomics data. In this study, we performed in vivo sampling using solid-phase ... -
Optimization of SPME coating characteristics for metabolomics and targeted analysis with LC/MS
(University of Waterloo, 2015-09-23)Metabolomics data provides complementary information to proteomics, genomics, and transcriptomics, in addition to enabling the tracking of the dynamic reactions in living systems. Metabolomics is widely used in various ... -
Supplementation Strategies for Tuning Glycosylation of Monoclonal Antibodies and Enhancing Growth in Mammalian Cell Culture by Omics Analysis
(University of Waterloo, 2018-04-17)Two fundamental objectives of bioprocess engineering are to increase productivity and improve product quality. Glycosylation is a critical and variable factor in the quality of several therapeutic proteins, particularly ...