Tupling, Allan Russell2006-07-282006-07-2820002000http://hdl.handle.net/10012/581The primary objective for this thesis was to investigate the changes that occur in the regulation of intracellular Ca2+ ([Ca2+]f) in skeletal muscle by the sarcoplasmic reticulum (SR) during ischemia (I) and ischemia-reperfusion (IR). In particular, the effects of I and IR on SR Ca2+ uptake, Ca2+ release and Ca2+-ATPaseactivity, measured in homogenates and isolated SR vesicles in vitro, were determined. In addition, the fluorescent probes, fluorescein isothiocyanate (FITC) and [(N-cyclohexyl-N'-dimethylamino-a-napthyl)carbodiimide] (NCD-4) , were used to measure structural alterations to the ATP binding site and the Ca2+ binding sites of the CA2+-ATPase, respectively, with I and IR. In the first study, an in vivo model of prolonged I in rat skeletal muscle was employed. To induce total I, a tourniquet was placed around the upper hindlimb in 30 female Sprague-Dawley rats, weighing 256 +_ 6.7g (mean +_ SE), and inflated to 350 mmHg for 4 hours.application/pdf5081158 bytesapplication/pdfenCopyright: 2000, Tupling, Allan Russell. All rights reserved.Harvested from Collections CanadaDoctoral Thesis