Kingston, David C.Acker, Stacey M.2018-11-142018-11-142018-12-01https://dx.doi.org/10.1016/j.jelekin.2018.08.006http://hdl.handle.net/10012/14123The final publication is available at Elsevier via https://dx.doi.org/10.1016/j.jelekin.2018.08.006 © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/Activation waveforms of vastus intermedius, adductor magnus, and semimembranosus have not been reported for high knee flexion activities such as kneeling or squatting, likely due to the invasive procedures required for their measurement. Their relatively large physiological cross sectional areas would suggest their contributions to knee joint loading could be considerable. Therefore, the purpose of this study was to quantify the activities of these muscles using fine-wire EMG and to assess easy to measure surface sites (vastus lateralis, rectus femoris, vastus medialis, semitendinosus, and biceps femoris) for their potential as proxy measures using <10 %MVC RMS and >0.85 R2 as criteria for successful representation of deep muscle activity by that measured at a surface site. Overall, no surface and fine-wire site pair met both criteria for these movements. When fine-wire measurement of muscle activity is infeasible or impractical, the waveforms presented in supplementary material could be used as a guide for the activity of these deep muscles. Although select muscles for some participants satisfied our criteria, inter-participant variability was considerable. Therefore, future muscle models may benefit from fine-wire measurement of these muscles, but researchers should be cautious of electrode site specificity.enAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/ElectromyographyFine-wireIndwellingKneelingSquattingArticle