Muraih, Jawad K.Palmer, Michael2017-04-132017-04-132012-07https://doi.org/10.1016/j.bbamem.2012.02.019http://hdl.handle.net/10012/11658The final publication is available at Elsevier via http://doi.org/10.1016/j.bbamem.2012.02.019 © 2012. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/Daptomycin is a lipopeptide antibiotic that kills Gram-positive bacteria by depolarizing their cell membranes. This antibacterial action of daptomycin is correlated with the formation of membrane-associated oligomers. We here examine the number of subunits contained in one oligomer using fluorescence resonance energy transfer (FRET). The results suggest that the oligomer contains approximately 6 to 7 subunits, or possibly twice this number if it spans both membrane monolayers.enAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Antibiotic daptomycinBactericidal activityBindingDaptomycinEnergy-transferFluorescence resonance energy transferLipopeptide antibioticsOligomer stoichiometryStaphylococcus-aureusArticle