Browsing Waterloo Research by Subject "Fluorescence"
Now showing items 1-19 of 19
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Cardiolipin Prevents Membrane Translocation and Permeabilization by Daptomycin
(American Society for Biochemistry and Molecular Biology, 2014-04-25)Daptomycin is an acidic lipopeptide antibiotic that, in the presence of calcium, forms oligomeric pores on membranes containing phosphatidylglycerol. It is clinically used against various Gram-positive bacteria such as ... -
Characterization of the Distribution of Pyrene Molecules in Confined Geometries with the Model Free Analysis
(American Chemical Society, 2017-12-21)Evidence is provided showing that global Model Free Analysis (MFA) of monomer and excimer fluorescence decays of pyrene dissolved in aqueous solutions of sodium dodecyl sulfate (SDS) provides the same structural and dynamic ... -
Chemical Modification of Polyisobutylene Succinimide Dispersants and Characterization of Their Associative Properties
(American Chemical Society, 2015-09-17)The secondary amines found in b-PIBSI dispersants prepared by attaching two polyisobutylene chains to a polyamine core via two succinimide moieties were reacted with ethylene carbonate (EC). The reaction generated urethane ... -
Comparison of Graphene Oxide and Reduced Graphene Oxide for DNA Adsorption and Sensing
(American Chemical Society, 2016-10-18)Fluorescently labeled DNA adsorbed on graphene oxide (GO) is a well-established sensing platform for detecting a diverse range of analytes. GO is a loosely defined material and its oxygen content may vary depending on the ... -
Daptomycin forms cation- and size-selective pores in model membranes
(Elsevier, 2014-10)Daptomycin is a lipopeptide antibiotic that is used clinically to treat severe infections caused by Gram-positive bacteria. Its bactericidal action involves the calcium-dependent binding to membranes containing ... -
Degradable starch nanoparticle assisted ethanol precipitation of DNA
(Elsevier, 2014-04-18)Precipitation of DNA from a large volume of aqueous solution is an important step in many molecular biology and analytical chemistry experiments. Currently, this is mainly achieved by ethanol precipitation, where a long-term ... -
DNA stabilized fluorescent metal nanoclusters for biosensor development
(Elsevier, 2014-03-22)Fluorescent silver, gold and copper nanoclusters (NCs) have emerged for biosensor development. Compared to semiconductor quantum dots, there is less concern about the toxicity of metal NCs, which can be more easily conjugated ... -
Efficient Triplet Exciton Fusion in Molecularly Doped Polymer Light-Emitting Diodes
(Wiley, 2017-04-04)Solution-processed polymer organic light-emitting diodes (OLEDs) doped with triplet-triplet annihilation (TTA)upconversion molecules, including 9,10-diphenylanthracene, perylene, rubrene and TIPS-pentacene, are reported. ... -
Electrostatically Directed Visual Fluorescence Response of DNA-Functionalized Monolithic Hydrogels for Highly Sensitive Hg2+ Detection
(American Chemical Society, 2011-03-23)Hydrogels are cross-linked hydrophilic polymer networks with low optical background and high loading capacity for immobilization of biomolecules. Importantly, the property of hydrogel can be precisely controlled by changing ... -
Fluorescent sensors using DNA-functionalized graphene oxide
(Springer, 2014-07-02)In the past few years, graphene oxide (GO) has emerged as a unique platform for developing DNA-based biosensors, given the DNA adsorption and fluorescence-quenching properties of GO. Adsorbed DNA probes can be desorbed ... -
Immobilization of DNA on Magnetic Microparticles for Mercury Enrichment and Detection with Flow Cytometry
(Wiley, 2011-04-26)Mercury detection in water has attracted a lot of research interest due to its highly toxic nature and adverse environmental impact. In particular, the recent discovery of specific binding of HgII to thymine-rich (T-rich) ... -
Label-Free Ag+ Detection by Enhancing DNA Sensitized Tb3+ Luminescence
(Multidisciplinary Digital Publishing Institute, 2016-08-26)In this work, the effect of Ag+ on DNA sensitized Tb3+ luminescence was studied initially using the Ag+-specific RNA-cleaving DNAzyme, Ag10c. While we expected to observe luminescence quenching by Ag+, a significant ... -
Mutual inhibition through hybrid oligomer formation of daptomycin and the semisynthetic lipopeptide antibiotic CB-182,462
(Elsevier, 2013-02)Daptomycin is a clinically important lipopeptide antibiotic that kills Gram-positive bacteria through membrane depolarization. Its activity requires calcium and the presence of phosphatidylglycerol in the target membrane. ... -
Partial oligomerization of pyolysin induced by a disulfide-tethered mutant
(NRC Research Press, 2012-12)The bacterial toxin pyolysin (PLO) belongs to the family of cholesterol-dependent cytolysins (CDCs), which form large, ring-shaped oligomeric pores in cholesterol-containing membranes. Monomeric CDC molecules have a structure ... -
Pyrenyl Derivative with a Four-Atom Linker That Can Probe the Local Polarity of Pyrene-Labeled Macromolecules
(American Chemical Society, 2016-02-04)The fluorescent probe 1-pyrenemethoxyethanol (PyMeEGOH) was designed to replace commercially available 1-pyrenebutanol (PyButOH) as an alternative fluorescent label to probe the internal dynamics and interior polarity of ... -
Quantifying the Level of Intermacromolecular Interactions in Ethylene-Propylene Copolymers by Using Pyrene Excimer Formation
(American Chemical Society, 2015-07-14)A unique methodology based on fluorescence measurements is introduced to quantitatively measure the actual level of interpolymeric association between ethylene-propylene (EP) copolymers used as viscosity index improvers ... -
Thermodynamics and Kinetics of Adaptive Binding in the Malachite Green RNA Aptamer
(American Chemical Society, 2013-09-24)Adaptive binding, the ability of molecules to fold themselves around the structure of a ligand and thereby incorporating it into their three-dimensional fold, is a key feature of most RNA aptamers. The malachite green ... -
Two successive calcium-dependent transitions mediate membrane binding and oligomerization of daptomycin and the related antibiotic A54145
(Elsevier, 2016-09)Daptomycin and A54145 are homologous lipopeptide antibiotics that permeabilize the cell membranes of Gram-positive bacteria. Membrane permeabilization depends on the presence of both phosphatidylglycerol (PG) and calcium, ... -
Visual optical biosensors based on DNA-functionalized polyacrylamide hydrogels
(Elsevier, 2013-12-15)Biosensors are devices that can provide quantitative or semi-quantitative analytical information about target molecules, where molecular recognition is based on biomolecular interactions. In recent years, DNA has emerged ...