Browsing Waterloo Research by Author "Sokolenko, Stanislav"
Now showing items 1-4 of 4
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Construction and analysis of a genetically tuneable lytic phage display system
Nicastro, Jessica; Sheldon, Katlyn; El-zarkout, Farah A.; Sokolenko, Stanislav; Aucoin, Marc; Slavcev, Roderick (Springer, 2013-09-01)The Bacteriophage lambda capsid protein gpD has been used extensively for fusion polypeptides that can be expressed from plasmids in Escherichia coli and remain soluble. In this study, a genetically controlled dual expression ... -
A correction method for systematic error in 1H-NMR time-course data validated through stochastic cell culture simulation
Sokolenko, Stanislav; Aucoin, Marc (BioMed Central, 2015-09-04)Background: The growing ubiquity of metabolomic techniques has facilitated high frequency time-course data collection for an increasing number of applications. While the concentration trends of individual metabolites can ... -
Identifying model error in metabolic flux analysis - a generalized least squares approach
Sokolenko, Stanislav; Quattrociocchi, Marco; Aucoin, Marc (BioMed Central, 2016-09-13)Background: The estimation of intracellular flux through traditional metabolic flux analysis (MFA) using an overdetermined system of equations is a well established practice in metabolic engineering. Despite the continued ... -
Treating Cell Culture Media with UV Irradiation against Adventitious Agents: Minimal Impact on CHO Performance
Yen, Sandi; Sokolenko, Stanislav; Manocha, Bhavik; Blondeel, Eric J. M.; Aucoin, Marc; Patras, Ankit; Daynouri-Pancino, Farnaz; Sasges, Michael (Wiley, 2014-10-01)Sterility of cell culture media is an important concern in biotherapeutic processing. In large scale biotherapeutic production, a unit contamination of cell culture media can have costly effects. Ultraviolet (UV) irradiation ...