Browsing Chemistry by Supervisor "Meiering, Elizabeth"
Now showing items 1-12 of 12
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Adnectin Solubility and Dynamics
(University of Waterloo, 2019-10-28)Rapid growth of the global market for monoclonal antibodies (mAbs) has generated considerable interest in the development of alternative molecules that facilitate rapid discovery and manufacturing, while replicating the ... -
Cellular Inclusion Bodies: Structure and Mechanisms
(University of Waterloo, 2022-08-18)Inclusion bodies (IBs) are insoluble aggregate structures that commonly form upon overexpression of proteins in heterologous hosts with relevance to biomedicine and biotechnology. These structures are of broad interest in ... -
Characterizing the Dimer Interface and Thermal Unfolding of Reduced, Zinc Bound Superoxide Dismutase-1
(University of Waterloo, 2018-04-30)Superoxide dismutase 1 (SOD1) is a ubiquitously expressed metalloenzyme that reduces oxidative stress in cells by catalyzing the dismutation of superoxide radicals. Mutations in the gene encoding for SOD1 have been linked ... -
Computational Design of Protein Structure and Prediction of Ligand Binding
(University of Waterloo, 2016-08-23)Proteins perform a tremendous array of finely-tuned functions which are not only critical in living organisms, but can be used for industrial and medical purposes. The ability to rationally design these molecular machines ... -
Engineering the kinetic stability of a β-trefoil protein by tuning its topological complexity
(University of Waterloo, 2022-10-03)Kinetic stability is crucially important for engineering stable proteins suitable for use in industrial, research, and medical applications. Specifically, kinetic stability is central to determining a protein’s functional ... -
Exploration of reconstructed PET hydrolase ancestors by biophysical and computational methods
(University of Waterloo, 2023-01-26)Ancestral sequence reconstruction (ASR), originally developed for the study of molecular evolution, has gained attention as a promising method for protein engineering in the recent decade. The ancestors revived by this ... -
Immature SOD1: protein folding, misfolding and aggregation
(University of Waterloo, 2023-01-25)Protein misfolding and aggregation are hallmarks of many diseases, including amyotrophic lateral sclerosis (ALS). In familial ALS, aberrant self-association of mutant Cu,Zn-superoxide dismutase (SOD1) is implicated as a ... -
Investigation of Immature Cu,Zn Superoxide Dismutase 1 Using Variable Temperature NMR
(University of Waterloo, 2020-09-03)Amyotrophic lateral sclerosis (ALS) is an adult onset neurodegenerative disease that results in the death of motor neurons in the spinal cord, cortex, and brain stem. The vast majority of ALS cases arise sporadically, but ... -
Stability and Strain in Hisactophilin and Mechanism of the Myristoyl Switch
(University of Waterloo, 2019-05-23)Hisactophilin is a myristoylated, histidine-rich, pH-dependent actin- and membrane-binding protein. In response to cellular changes in pH, this β-trefoil protein reversibly switches between cytosolic and membrane- bound ... -
Structural characterization and engineering of Adnectin inclusion bodies
(University of Waterloo, 2022-08-15)Protein aggregation is a fundamental process occurring as a response to aging, disease, protein overexpression and in many areas of biotechnology and medical applications. There has been much recent progress in determining ... -
Structural characterization of f-ALS associated SOD1 mutant aggregates using conformation-specific antibodies
(University of Waterloo, 2020-06-01)Superoxide dismutase 1 (SOD1) is a metalloenzyme ubiquitously expressed in all cells and acts as an antioxidant enzyme by catalyzing the dismutation of superoxide radicals. Several mutations in the gene encoding SOD1 are ... -
Structure of Human Cu, Zn Superoxide Dismutase-1 Inclusion Body Aggregates using Quenched Hydrogen-Deuterium Amide Exchange
(University of Waterloo, 2021-12-22)Inclusion bodies (IBs) are insoluble protein aggregates that can be formed within recombinant bacteria. These aggregates, once thought to be an obstacle in recombinant protein expression and purification, have recently ...