Browsing Chemistry by Title
Now showing items 61-80 of 826
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Bacteriophage M13 as a Template for Multi-Enzyme Assembly
(University of Waterloo, 2019-09-25)An important aspect of bionanotechnology is the utilization of biomolecules to prepare controlled and defined arrangements of multiple components with nanoscale order. Bacteriophage M13 is a filamentous phage composed of ... -
Binding of Au3+ Ions by Polyadenine DNA
(University of Waterloo, 2020-09-02)Interactions between DNA and metal ions are important for many applications such as metal sensing, therapeutics and nanotechnology. Many studies have already been published on these topics. DNA can bind metal ions via its ... -
Biochemical and Biophysical Investigations of Non-Zinc Dependent Glyoxalase I Enzymes
(University of Waterloo, 2008-09-26)The principal methylglyoxal (MG)-detoxifying system in most living organisms is the two metalloenzyme Glyoxalase system. Glyoxalase I (GlxI) initially converts the non-enzymatically formed MG-GSH hemithioacetal to the ... -
Biochemical and Microbiological Investigations of Inhibitors for β-Lactamases from Human Pathogenic Bacteria
(University of Waterloo, 2019-04-30)The majority of antibiotics prescribed for treatment of bacterial infections are β-lactam antibiotics. Resistance to these has evolved in a few different ways, notably by regulating permeability and by the expression of ... -
Biochemical Characterization and Optimization of RNA Aptamers Bound to Hoechst Dye Derivatives
(University of Waterloo, 2023-08-09)Ribonucleic acid (RNA) aptamers are single-stranded nucleic acids that are typically selected through a process known as Systematic Evolution of Ligands by EXponential Enrichment (SELEX). These oligonucleotides can bind ... -
Biochemical Characterization of a Lanthanide-Dependent DNAzyme with Normal and Phosphorothioate-Modified Substrates
(American Chemical Society, 2015-10-06)A trivalent lanthanide (Ln3+)-dependent RNA-cleaving DNAzyme, Ce13d, was recently isolated via in vitro selection. Ce13d is active in the presence of all Ln3+ ions. Via introduction of a single phosphorothioate (PS) ... -
Biochemical Characterization of Fluorophore-Binding Nucleic Acids
(University of Waterloo, 2022-09-27)Aptamers, single stranded RNA or DNA that bind to target ligands with high affinity and specificity, have a wide range of applications, from therapeutics to biosensing, which makes biochemical characterization of the utmost ... -
Biochemical Investigations of L-Methionine gamma-lyase 1 from Trichomonas vaginalis
(University of Waterloo, 2011-12-16)The enzyme L-methionine γ-lyase (MGL) utilizes a pyridoxal-5’-phosphate-cofactor in order to convert L-methionine to α-ketobutyrate, ammonia and methyl mercaptan. MGL is proposed to be a potential drug target since it is ... -
Biochemistry in Bacterioferritin
(University of Waterloo, 2006)Bacterioferritin, an iron storage protein having a 24-subunit quaternary structure, was used as a model for the study of host-guest interactions and guest encapsulation, making use of its spherical cage-like structure. ... -
BIOCOMPATIBLE SOLID PHASE MICROEXTRACTION
(University of Waterloo, 2006-12-12)Today’s solid phase microextraction (SPME) is a well known technique that combines knowledge from different fields in an attractive, efficient, and economic way. The development of SPME has seen huge growth since its ... -
Biocompatible Solid-Phase Microextraction Nanoelectrospray Ionization: An Unexploited Tool in Bioanalysis
(American Chemical Society, 2015-12-09)In recent years, different geometrical configurations of solid-phase microextraction (SPME) have been directly coupled to mass spectrometry, resulting in benefits such as diminishing matrix effects, improvement of detection ... -
Biological responses in fish exposed to municipal wastewater treatment plant effluent in situ
(IWA Publishing, 2021-04-16)Effluents from municipal wastewater treatment plants (MWTPs) are complex mixtures of chemicals including endocrine-disrupting compounds (EDCs) and 17α-ethynylestradiol (EE2). The objective of this study was to evaluate ... -
Biomimetic sensing based on chemically induced assembly of a signaling DNA aptamer on a fluid bilayer membrane
(Royal Society of Chemistry, 2012-04-18)The adenosine aptamer was split into two halves and linked to a fluid liposome surface; addition of adenosine resulted in aptamer assembly, which did not occur if the split aptamer was attached to silica nanoparticles, ... -
Bioorthogonal Modification of the Major Sheath Protein of Bacteriophage M13: Extending the Versatility of Bionanomaterial Scaffolds
(American Chemical Society, 2016-09-04)With a mass of ~1.6 x 107 Daltons and com- posed of approximately 2700 proteins, bacteriophage M13 has been employed as a molecular scaffold in bionanomaterials fabrication. In order to extend the versatility of M13 in ... -
Biophysical Investigations of the Interactions between Calmodulin and Nitric Oxide Synthase Enzymes
(University of Waterloo, 2017-12-15)Nitric Oxide (•NO) is an essential biomolecule that has physiological functions in neurotransmission, vasodilation and immune response. •NO is produced by a family of enzymes known as nitric oxide synthases (NOS). NOS ... -
Biosensing at an individually addressable electrochemical array
(University of Waterloo, 2006)In this thesis, a novel electrochemical array is reported. The array consists of two planar halves, each having four carbon screen-printed band electrodes (SPEs), orthogonally facing each other and separated by a spacer ... -
Blue emitting gold nanoclusters templated by poly-cytosine DNA at low pH and poly-adenine DNA at neutral pH
(Royal Society of Chemistry, 2012-07-11)Blue fluorescent gold nanoclusters were prepared in the presence of poly-cytosine DNAs at low pH and poly-adenine at neutral pH using citrate as the reducing agent; various buffer conditions affecting the synthesis have ... -
Boosting the oxidase mimicking activity of nanoceria by fluoride capping: rivaling protein enzymes and ultrasensitive F- detection
(Royal Society of Chemistry, 2016-07-28)Nanomaterial-based enzyme mimics (nanozymes) are currently a new forefront of chemical research. However, the application of nanozymes is limited by their low catalytic activity and low turnover numbers. Cerium dioxide ... -
Bulk Supramolecular Polymerization via “Weak” Hydrogen Bonds and Aromatic Interactions: Recognizing the Potential of Metal Coordination Geometry
(University of Waterloo, 2019-01-25)Bulk supramolecular polymers offer an attractive strategy for the development of functional materials. The use of small molecule monomers is of particular interest, because they maximize the potential of a fully reversible ... -
Cage-like Proteins as Bioink Components for 3D Bioprinting
(University of Waterloo, 2018-01-24)Hydrogel matrices have been used as structural surrogates in 3D bioprinting as a mechanism to provide the appropriate environment for cell adhesion and proliferation. In this research, the preparation and optimization of ...