Now showing items 1-10 of 118
Dissecting the effect of anions on Hg2+ detection using a FRET based DNA probe
(Royal Society of Chemistry, 2012-08-07)
Many biosensors have been developed to detect Hg2+ using thymine-rich DNA. While sensor response to various cations is often studied to demonstrate selectivity, the effect of anions has been largely overlooked. Anions may ...
DNA adsorption by magnetic iron oxide nanoparticles and its application for arsenate detection
(Royal Society of Chemistry, 2014-05-01)
Iron oxide nanoparticles adsorb fluorescently labeled DNA oligonucleotides via the backbone phosphate and quench fluorescence. Arsenate displaces adsorbed DNA to increase fluorescence, allowing detection of arsenate down ...
In Vitro Selection of a New Lanthanide-Dependent DNAzyme for Ratiometric Sensing Lanthanides
(American Chemical Society, 2014-10-07)
Developing biosensors for lanthanides is an important but challenging analytical task. To address this problem, in vitro selection of RNA-cleaving DNAzymes was carried out using a library containing a region of 35 random ...
The group trend of lanthanides binding to DNA and DNAzymes with a complex but symmetric pattern
(Royal Society of Chemistry, 2014-07-18)
Using sensitized Tb3+ luminescence spectroscopy as a tool, binding of 14 lanthanides to a lanthanide-dependent DNAzyme is studied, where the binding affinity is symmetric across the series and the tightest binding occurs ...
Aptamer-Functionalized Hydrogel Microparticles for Fast Visual Detection of Mercury(II) and Adenosine
(American Chemical Society, 2012-04-25)
With a low optical background, high loading capacity, and good biocompatibility, hydrogels are ideal materials for immobilization of biopolymers to develop optical biosensors. We recently immobilized mercury and lead binding ...
Instantaneous and Quantitative Functionalization of Gold Nanoparticles with Thiolated DNA Using a pH-Assisted and Surfactant-Free Route
(American Chemical Society, 2012-05-02)
The attachment of thiolated DNA to gold nanoparticles (AuNPs) has enabled many landmark works in nanobiotechnology. This conjugate chemistry is typically performed using a salt-aging protocol where, in the presence of an ...
Molecular Beacon Lighting up on Graphene Oxide
(American Chemical Society, 2012-05-01)
A molecular beacon (MB) is comprised of a fluorophore and a quencher linked by a DNA hairpin. MBs have been widely used for homogeneous DNA detection. In addition to molecular quenchers, many nanomaterials such as graphene ...
Surface Science of DNA Adsorption onto Citrate-Capped Gold Nanoparticles
(American Chemical Society, 2012-02-28)
Single-stranded DNA can be adsorbed by citrate capped gold nanoparticles (AuNPs), resulting in increased AuNP stability, which forms the basis of a number of biochemical and analytical applications, but the fundamental ...
DNA-Length-Dependent Fluorescence Signaling on Graphene Oxide Surface
Fluorescence energy transfer to graphene oxide is studied using covalently linked DNA probes ranging from 4 to 70 base pairs. The characteristic distance and mechanism of energy transfer are reported.
Ultrahigh Nanoparticle Stability against Salt, pH, and Solvent with Retained Surface Accessibility via Depletion Stabilization
(American Chemical Society, 2012-06-20)
For many applications, it is desirable to stabilize colloids over a wide range of buffer conditions while still retaining surface accessibility for adsorption and reaction. Commonly used charge or steric stabilization ...