Now showing items 1-10 of 41
DNA-Length-Dependent Fluorescence Signaling on Graphene Oxide Surface
Fluorescence energy transfer to graphene oxide is studied using covalently linked DNA probes ranging from 4 to 70 base pairs. The characteristic distance and mechanism of energy transfer are reported.
In Vitro Selection of a DNAzyme Cooperatively Binding Two Lanthanide Ions for RNA Cleavage
(American Chemical Society, 2016-05-03)
Trivalent lanthanide ions (Ln(3+)) were recently employed to select RNA-cleaving DNAzymes, and three new DNAzymes have been reported so far. In this work, dysprosium (Dy3+) was used with a library containing 50 random ...
DNA Adsorption by ZnO Nanoparticles near Its Solubility Limit: Implications for DNA Fluorescence Quenching and DNAzyme Activity Assays
(American Chemical Society, 2016-06-07)
Zinc oxide (ZnO) is a highly important material, and Zn2+ is a key metal ion in biology. ZnO and Zn2+ interconvert via dissolution and hydrolysis/condensation. In this work, we explore their interactions with DNA, which ...
Distinction of Individual Lanthanide Ions with a DNAzyme Beacon Array
(American Chemical Society, 2016-06-24)
Developing chemical probes to distinguish each lanthanide ion is a long-standing challenge. Aside from its analytical applications, solving this problem will also enhance our knowledge in metal ligand design. Using in vitro ...
Hg2+ detection using a phosphorothioate RNA probe adsorbed on graphene oxide and a comparison with thymine-rich DNA
(Royal Society of Chemistry, 2016-06-21)
Mercury is a highly toxic heavy metal and many DNA-based biosensors have been recently developed for Hg2+ detection in water. Among them, thymine-rich DNA is the most commonly used for designing Hg2+ sensors. However, the ...
In Vitro Selection of New DNAzymes as Metal Biosensors
(University of Waterloo, 2015-06-26)
In vitro selection of metal-specific DNAzymes has been shown to be a powerful method to obtain biosensors for metal ion detection. With 14 independent in vitro selection experiments using each of the trivalent lanthanide ...
Nucleotide coordination with 14 lanthanides studied by isothermal titration calorimetry
With their hydrolytic, optical and magnetic properties, lanthanide ions (Ln3+) are versatile probes for nucleic acids. In addition, nucleotide-coordinated Ln3+ ions form useful nanoparticles. However, the thermodynamic ...
A DNAzyme requiring two different metal ions at two distinct sites
(Oxford University Press, 2015-12-10)
Most previously reported RNA-cleaving DNAzymes require only a single divalent metal ion for catalysis. We recently reported a general trivalent lanthanide-dependent DNAzyme named Ce13d. This work shows that Ce13d requires ...
G-Quadruplex DNA for Fluorescent and Colorimetric Detection of Thallium(I)
(American Chemical Society, 2016-02-26)
Thallium is a highly toxic heavy metal, but its 495 nm FRET sensing is underexplored compared to its neighboring elements Blue in the periodic table: lead and mercury. Thallium has two " oxidation states. A DNAzyme-based ...
Liposome/Graphene Oxide Interaction Studied by Isothermal Titration Calorimetry
(American Chemical Society, 2015-03-15)
The interaction between graphene oxide (GO) and lipid bilayers is important for fundamental surface science and many applications. In this work, isothermal titration calorimetry (ITC), cryo-TEM, and fluorescence spectroscopy ...