Browsing Science (Faculty of) by Supervisor "Duncker, Bernard"
Now showing items 1-7 of 7
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The characterization of Dbf4 interactions and roles in genome replication and stability in Saccharomyces cerevisiae
(University of Waterloo, 2020-03-05)Dbf4-dependent kinase (DDK) is a complex composed of the Cdc7 kinase and its regulatory subunit, Dbf4. Initiation of eukaryotic DNA replication requires phosphorylation of Mcm2-7 helicase by DDK. Due to its essential role ... -
Characterization of Mrc1 during Replication Initiation in Saccharomyces cerevisiae
(University of Waterloo, 2022-01-12)DNA replication is a highly specific process that is completed under the control of several factors, to ensure faithful duplication of the genome. Mrc1 (Claspin in mammalian cells) is a protein that is a part of a fork ... -
Characterization of novel FHA domain ligand binding motifs
(University of Waterloo, 2016-01-25)Rad53, a key checkpoint protein in Saccharomyces cerevisiae, has a central catalytic kinase domain as well as two forkhead-associated (FHA) domains, FHA1 and FHA2. Along with the extensively studied phosphothreonine binding ... -
Characterization of the FHA domain involved in Saccharomyces cerevisiae dNTP regulation.
(University of Waterloo, 2018-04-26)Over 2000 Forkhead-associated (FHA) domain-containing proteins exhibiting diverse functions, such as kinases, phosphatases, and transcription factors, have been identified to date in both eukaryotic and prokaryotic organisms. ... -
Characterization of the Mechanistic Roles of Rif1-Glc7 in DNA Double-Strand Break Repair Pathway Choice in Saccharomyces cerevisiae
(University of Waterloo, 2023-05-10)Rap-1 interacting factor 1 (Rif1) is a protein involved in telomere regulation, DNA replication and has more recently been found to mediate genomic stability by influencing the choice of DNA double-strand break (DSB) repair ... -
The characterization of γH2AX as a biomarker of genotoxicity in Oncorhynchus mykiss
(University of Waterloo, 2017-05-19)Environmental contamination due to by-products and waste from industrial activities is a growing universal concern, especially regarding the impact on water and the aquaculture industry. Conventional tests to diagnose ... -
Investigating the involvement of FHA domain lateral surfaces in DNA damage and cell cycle checkpoint responses in Saccharomyces cerevisiae
(University of Waterloo, 2023-08-30)Since the discovery of the Forkhead-associated (FHA) domain in 1995, research groups have pursued the characterization of FHA domains and the interactions that they regulate through the lens of their pThr-epitope binding ...